Molecular Imaging of Apoptosis with Radio-Labeled Annexin A5 Focused on the Evaluation of Tumor Response to Chemotherapy
Molecular Imaging of Apoptosis with Radio-Labeled Annexin A5 Focused on the Evaluation of Tumor Response to Chemotherapy
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DOI：
10.2174/187152009789377736
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发表时间：
2009-11-01
影响因子：
2.8
通讯作者：
Tamaki, Nagara
中科院分区：
文献类型：
--
作者：
Kuge, Yuji;Zhao, Songji;Tamaki, Nagara
细胞凋亡或程序性细胞死亡在成功的抗肿瘤治疗过程中被激活。确定细胞凋亡的基线水平和治疗诱导的细胞凋亡的增量可以作为有用的预后标志物。因此，细胞凋亡的非侵入性评估将为临床医生提供治疗效果以及新抗癌药物的开发和测试的信息。在这些方面，细胞凋亡检测放射性探针（放射性药物）已被广泛研究。膜联蛋白 A5（膜联蛋白 V）是一种内源性蛋白质，以高亲和力和特异性与磷脂酰丝氨酸结合，磷脂酰丝氨酸在细胞凋亡的早期过程中呈现在细胞表面。因此，可以使用标记有放射性核素（例如Tc-99m和F-18）的膜联蛋白A5在体内检测凋亡细胞。迄今为止，已开发出几种膜联蛋白 A5 无线电探针。其中，Tc-99m-HYNIC-annexin A5 是细胞凋亡成像的最佳候选者。使用放射性标记的膜联蛋白 A5 的细胞凋亡成像已应用于检测体内细胞凋亡，用于肿瘤对化疗或放疗反应的实验和临床评估。本综述描述了使用膜联蛋白 A5 放射性探针进行细胞凋亡成像，重点关注其在评估肿瘤对化疗的反应中的应用。首先，描述了使用膜联蛋白 A5 放射性探针进行细胞凋亡成像的原理。接下来，讨论放射性标记的膜联蛋白 A5 在疗效评估中的实验结果。最后，讨论了放射性标记的膜联蛋白 A5 凋亡成像的临床应用。
Apoptosis, or programmed cell death, is activated in the course of successful anti-neoplastic therapy. Determining baseline levels of apoptosis and the increment of apoptosis induced by therapy can serve as useful prognostic markers. Thus, non-invasive assessment of apoptosis would be desirable to provide clinicians with information on therapeutic efficacy as well as for the development and testing of new anticancer drugs. In these regards, apoptosis detecting radio-probes (radiopharmaceuticals) have been extensively studied. Annexin A5 (annexin V) is an endogenous protein that binds with high affinity and specificity to phosphatidylserine, which is presented on the cell surface in an early process of apoptosis. Accordingly, apoptotic cells can be detected in vivo using annexin A5 labeled with radionuclides, such as Tc-99m and F-18. To date, several annexin A5 radio-probes have been developed. Among these, Tc-99m-HYNIC-annexin A5 is the best candidate for apoptosis imaging. The apoptosis imaging using radio-labeled annexin A5 has been applied for detecting apoptosis in vivo in the experimental and clinical evaluation of the tumor response to chemotherapy or radiotherapy. The present review describes apoptosis imaging with annexin A5 radio-probes, focusing on its application to the evaluation of the tumor response to chemotherapy. First, principles of apoptosis imaging with annexin A5 radio-probes are described. Next, experimental results with radio-labeled annexin A5 in the evaluation of therapeutic efficacy are discussed. Finally, clinical application of apoptosis imaging with radio-labeled annexin A5 is addressed.