Characterization of eleven antigenic groups in Trichinella genus and identification of stage and species markers
Characterization of eleven antigenic groups in Trichinella genus and identification of stage and species markers
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DOI：
10.1017/s0031182097001716
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发表时间：
1997-12-01
期刊：
影响因子：
2.4
通讯作者：
Soulé, C
中科院分区：
文献类型：
--
作者：
Boireau, P;Vayssier, M;Soulé, C
针对旋毛虫 (Ts) 和假旋毛虫 (Tp) 的 L1 肌肉阶段 (L1M) 产生了几种单克隆抗体 (Mab)。对各种抗原制剂的蛋白质印迹分析表明，不同作者描述的 Mab 可识别感染性幼虫粗提物中的 8 个抗原级分 (TSL1-TSL8)。 TSL1 部分具有免疫显性，存在于不同寄生虫阶段的角质层上。选择抗旋毛虫 T5 (T5) 和 Ts 的单克隆抗体是为了将之前的研究扩展到另一种旋毛虫表型。在斑点印迹程序和 ELISA 测试中，所选 Mab 中只有 35% 识别线性表位，71% 与可溶性或排泄性抗原发生反应。 Mab 的靶标通过使用 [S-35] 蛋氨酸标记的 L1M 蠕虫裂解物进行免疫沉淀来鉴定。由用完整寄生虫（T5）免疫的小鼠制备的单克隆抗体可识别不同寄生虫器官中更广泛的抗原。在角质层上识别出七种抗原结构，并在肠道、血淋巴和粘红细胞中鉴定出几个表位。根据蠕虫横截面的间接免疫荧光模式建立了十一个抗原组。针对 Ts 可溶性抗原产生的单克隆抗体主要识别针状细胞和角质层表面的表位。所有选定的 Mab 均识别出 T5 和布里托维旋毛虫 (Tb)，加强了这两个物种之间的联系。四种 Mab 用于区分 6 种旋毛虫表型之间的抗原结构，并开发了一种新工具来追踪旋毛虫属内的基因流。
Several monoclonal antibodies (Mabs) were raised against the L1 muscle stage (L1M) of Trichinella spiralis (Ts) and Trichinella pseudospiralis (Tp). Western blot analysis of various antigenic preparations established that Mabs described by different authors recognized 8 antigenic fractions (TSL1-TSL8) in crude extracts of infective larvae. The TSL1 fraction was immunodominant and present on the cuticle of different parasite stages. Mabs against Trichinella T5 (T5) and Ts were selected in order to extend the previous studies to another Trichinella phenotype. Only 35%, of the selected Mabs recognized linear epitopes and 71%, reacted with soluble or excretory-secretory antigens in a dot blot procedure and ELISA test. The targets of the Mabs were identified by immunoprecipitation with [S-35]methionine-labelled L1M worm lysate. Mabs prepared from mice immunized with the whole parasite (T5) recognized a wider panel of antigens in different parasitic organs. Seven antigenic structures were distinguished on the cuticle and several epitopes were identified in the gut, haemolymph and stichocytes. Eleven antigenic groups were established according to their indirect immunofluorescence pattern on cross-sections of the worm. Monoclonal antibodies raised against Ts soluble antigen mainly recognized epitopes in stichocytes and on the cuticle surface. All the selected Mabs recognized T5 and Trichinella britovi (Tb) strengthening the link between these 2 species. Four Mabs were used to differentiate antigenic structures among 6 Trichinella phenotypes and to develop a new tool to follow gene flow within the Trichinella genus.