Altered expression of PIWI RNA in the rat brain after transient focal ischemia.
Altered expression of PIWI RNA in the rat brain after transient focal ischemia.
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DOI：
10.1161/strokeaha.110.598391
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发表时间：
2011-04
期刊：
影响因子：
8.3
通讯作者：
Vemuganti R
中科院分区：
文献类型：
--
作者：
Dharap A;Nakka VP;Vemuganti R
The piwi-interacting RNA (piRNA) is the most predominant RNA species in eukaryotes. The piRNA are a class of non-coding (nc) RNA that bind and degrade the RNA formed by the transposons to control the transposon -induced gene mutations. The role of piRNA after focal ischemia is not yet evaluated. We profiled 39,727 piRNAs in the cerebral cortex of adult rats subjected to transient focal ischemia using microarrays. The RT targets of stroke-responsive piRNAs were identified with bioinformatics. To understand how piRNA are controlled, we analyzed the transcription factor (TF) binding sites in the putative promoters of 10 representative stroke-responsive piRNAs. In the ipsilateral cortex of ischemic rats, 105 piRNAs showed altered expression (54 up- and 51 down-regulated; >2.5 fold) compared to sham. Twenty five of those showed >5-fold change. A bioinformatics search showed that the transposon targets of the highly stroke-responsive piRNAs are distributed among the 20 autosomal chromosomes and there is a redundancy in the targets between the piRNAs. Furthermore, the transposon targets were observed to be highly repetitious for each piRNA across the chromosome length. Of the 159 TFs observed to have binding sites in the piRNA gene promoters, 59% belonged to 20 major families indicating that TFs control stroke-responsive piRNAs in a redundant manner. The present study is the first to show that many piRNAs are expressed in adult rodent brain and several of them respond to focal ischemia.
