Homology throughout the multiple 32-kilobase circular plasmids present in Lyme disease spirochetes
Homology throughout the multiple 32-kilobase circular plasmids present in Lyme disease spirochetes
复制标题
DOI：
10.1128/jb.179.1.217-227.1997
复制
发表时间：
1997-01-01
影响因子：
3.2
通讯作者：
Stevenson, B
中科院分区：
文献类型：
--
作者：
Casjens, S;vanVugt, R;Stevenson, B
我们已经鉴定了伯氏疏螺旋体B31株携带的7个不同的32kb环状质粒。限制性内切酶识别位点定位和部分测序表明，这7个质粒在整个长度上可能都与彼此紧密相关，并与伯氏杆菌晚期分离株IP21的8.3kb环状质粒cp8.3有很高的亲缘关系。加上7个32kb的质粒，该细菌菌株已知携带至少10个线形和9个环状质粒。缺乏一个或多个32kb环状质粒的伯氏杆菌B31的变种培养是活的，至少在某些情况下是有感染性的。我们检查了莱姆病疏螺旋体的一些不同的自然分离株，发现所有严格意义上的伯氏杆菌分离株和大部分测试的伯氏疏螺旋体晚期分离株似乎携带与伯氏疏螺旋体B31相关的多个32kb的环状质粒。这些质粒的普遍存在表明，它们可能在这些生物的自然生命周期中发挥着重要作用。它们可能是高度结合的质粒或原噬菌体基因组，这可能有助于对伯氏杆菌的遗传操作。
We have characterized seven different 32-kb circular plasmids carried by Borrelia burgdorferi isolate B31. Restriction endonuclease recognition site mapping and partial sequencing of these plasmids indicated that all seven are probably closely I elated to each other throughout their lengths and have substantial relationships to cp8.3, an 8.3-kb circular plasmid of B. burgdorferi sensu late isolate Ip21. With the addition of the seven 32-kb plasmids, this bacterial strain is known to carry at least 10 linear and 9 circular plasmids. Variant cultures of B. burgdorferi B31 lacking one or more of the 32-kb circular plasmids are viable and, at least in some cases, infectious. We have examined a number of different natural isolates of Lyme disease borreliae and found that all of the B. burgdorferi sensu stricto isolates and most of the B. burgdorferi sensu late isolates tested appear to carry multiple 32-kb circular plasmids related to those of B. burgdorferi B31. The ubiquity of these plasmids suggests that they may be important in the natural life cycle of these organisms. They may be highly conjugative plasmids or prophage genomes, which could prole to be useful in genetically manipulating B. burgdorferi.