Phylogenetic relationships of rat lungworm, Angiostrongylus cantonensis, isolated from different geographical regions revealed widespread multiple lineages
Phylogenetic relationships of rat lungworm, Angiostrongylus cantonensis, isolated from different geographical regions revealed widespread multiple lineages
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DOI：
10.1016/j.parint.2012.02.005
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发表时间：
2012-09-01
影响因子：
1.9
通讯作者：
Ohta, Nobuo
中科院分区：
文献类型：
--
作者：
Tokiwa, Toshihiro;Harunari, Tsunehito;Ohta, Nobuo
我们利用小亚基 (SSU) 核糖体 (r) RNA 和线粒体细胞色素 C 氧化酶亚基 I (cox!) 基因序列对广州黄芪的遗传变异进行了初步调查。在来自日本、中国大陆、台湾和泰国 17 个不同地理位置的 17 个广州曲霉个体中鉴定出两种不同的 SSU 基因型（C1 和 G2）。确定了来自日本、中国大陆、台湾和泰国 18 个不同地理位置的 83 种蠕虫的部分 coxl 序列。系统发育分析显示了八种不同的 coxl 单倍型（ad 至 ac8）。在 18 个地点中的 16 个地点中，仅发现了单一 coxl 单倍型。然而，在两个地点，两种coxl单倍型共存。常见的单倍型有：单倍型ad l（东京、千叶、神奈川、奄美大岛和台中）、单倍型ac2（石川、深圳和廉江）、单倍型ac5（冲绳和小笠原群岛）和单倍型ac7（宫城、爱知和神奈川）。这些区域中的每一个都被高山或海洋与其他区域分开。此外，广州曲霉在每个地点较低的遗传变异和特定的地理分布可能表明创始人效应，这可能是由多个独立起源造成的，并表明单倍型通过与人类相关的运输从流行地区迁移。 (C) 2012 Elsevier Ireland Ltd. 保留所有权利。
We conducted a pilot survey of genetic variation of A. cantonensis using small subunit (SSU) ribosomal (r) RNA and mitochondrial cytochrome c oxidase subunit I (cox!) gene sequences. Two distinct SSU genotypes (Cl and G2) were identified among 17 individual A. cantonensis worms from 17 different geographical localities in Japan, Mainland China, Taiwan, and Thailand. The partial coxl sequences were determined for 83 worms from 18 different geographical localities from Japan, Mainland China, Taiwan, and Thailand. Phylogenetic analysis showed eight distinct coxl haplotypes (ad to ac8). In 16 out of 18 localities, only a single coxl haplotype was found. However, in two localities, two coxl haplotypes coexisted. The common haplotypes found were: haplotype ad l (Tokyo, Chiba, Kanagawa, Amamioshima Island, and Taichung), haplotype ac2 (Ishikawa, Shenzhen, and Lianjiang), haplotype ac5 (the Okinawa and the Ogasawara Islands), and haplotype ac7 (Miyagi, Aichi, and Kanagawa). Each of these regions is separated from the others by high mountain ranges or oceans. In addition, the lower genetic variation and particular geographical distribution of A. cantonensis in each location could indicate a founder effect, which may have resulted from multiple independent origins, and suggests that haplotypes migrated from endemic areas via human-related transportation. (C) 2012 Elsevier Ireland Ltd. All rights reserved.