Distinct fatty acid signatures in infrapatellar fat pad and synovial fluid of patients with osteoarthritis versus rheumatoid arthritis
Distinct fatty acid signatures in infrapatellar fat pad and synovial fluid of patients with osteoarthritis versus rheumatoid arthritis
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DOI：
10.1186/s13075-019-1914-y
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发表时间：
2019-05-22
影响因子：
4.9
通讯作者：
Nieminen, Petteri
中科院分区：
文献类型：
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作者：
Mustonen, Anne-Mari;Kakela, Reijo;Nieminen, Petteri
背景：最近，膝下脂肪垫(IFP)已成为膝关节疾病炎症的潜在来源。它被认为是脂肪细胞因子、脂肪酸(FA)和FA衍生的脂质介质的来源之一，这些物质可能有助于膝关节的病理生理过程。滑液(SF)脂质成分的改变与骨性关节炎(OA)和类风湿性关节炎(RA)有关。本研究的目的是比较类风湿性关节炎(RA)和骨性关节炎(OA)患者在IFP和SF中的FA信号。方法：采集RA(n=10)和OA(n=10)行全关节置换手术的同侧膝关节的IFP和SF样本。对照SF样本(n=6)是在诊断或治疗性膝关节镜手术期间采集的，与RA或OA无关。结果：关节病引起的n-6多不饱和脂肪酸(PUFA)的SF比例显著降低，且以OA较RA更为明显。油酸的特征还包括22：6n-3的比例降低和n-3多不饱和脂肪酸的产物/前体比降低。总单不饱和脂肪酸在RA和OASF中所占比例均增加。对于IFP，RA患者的20：4n-6、总n-6PUFA和22：6n-3的比例以及n-3PUFA的产物/前体比率低于OA患者。在两种诊断中，SF FA的平均链长和OA的双键指数均降低。结论：FA信号的复杂变化可能是RA和OA膝关节炎症过程和软骨破坏的原因之一，但也可能是有限的。
Background: Infrapatellar fat pad (IFP) has recently emerged as a potential source of inflammation in knee arthropathies. It has been proposed to be one source of adipocytokines, fatty acids (FA), and FA-derived lipid mediators that could contribute to the pathophysiological processes in the knee joint. Alterations in synovial fluid (SF) lipid composition have been linked to both osteoarthritis (OA) and rheumatoid arthritis (RA). The aim of the present study was to compare the FA signatures in the IFP and SF of RA and OA patients.Methods: Pairs of IFP and SF samples were collected from the same knees of RA (n=10) and OA patients (n=10) undergoing total joint replacement surgery. Control SF samples (n=6) were harvested during diagnostic or therapeutic arthroscopic knee surgery unrelated to RA or OA. The FA composition in the total lipids of IFP and SF was determined by gas chromatography with flame ionization and mass spectrometric detection.Results: Arthropathies resulted in a significant reduction in the SF proportions of n-6 polyunsaturated FA (PUFA), more pronouncedly in OA than in RA. OA was also characterized with reduced percentages of 22:6n-3 and lower product/precursor ratios of n-3 PUFA. The proportions of total monounsaturated FA increased in both RA and OA SF. Regarding IFP, RA patients had lower proportions of 20:4n-6, total n-6 PUFA, and 22:6n-3, as well as lower product/precursor ratios of n-3 PUFA compared to OA patients. The average chain length of SF FA decreased in both diagnoses and the double bond index in OA.Conclusions: The observed complex alterations in the FA signatures could have both contributed to but also limited the inflammatory processes and cartilage destruction in the RA and OA knees.