The hydrodynamic radii of macromolecules and their effect on red blood cell aggregation
The hydrodynamic radii of macromolecules and their effect on red blood cell aggregation
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DOI：
10.1529/biophysj.104.047746
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发表时间：
2004-12-01
影响因子：
3.4
通讯作者：
Fisher, TC
中科院分区：
文献类型：
--
作者：
Armstrong, JK;Wenby, RB;Fisher, TC
研究了非离子聚合物对人红细胞（RBC）聚集的影响。利用爱因斯坦黏度关系法计算了葡聚糖、聚乙烯吡罗烷酮和聚氧乙烯在1500 ~ 200000分子量范围内的特性黏度，并通过准弹性光散射直接测量了它们的流体动力学半径（Rh），并通过浊度法和低剪切黏度法研究了每种聚合物样品对红细胞聚集的影响。对于这三种聚合物，尽管它们的结构不同，样品的R-h 4nm聚集增强。抑制作用随R-h的增加而增强，在；3海里;在4 nm以上，促聚效应随R-h的增加而增强。为了比较，我们根据文献中的特性黏度或扩散系数计算了12种血浆蛋白的R-h。已知促进红细胞聚集的每一种蛋白的R-h浓度均为40 nm，而那些具有R-h浓度的蛋白则为40 nm
The effects of nonionic polymers on human red blood cell (RBC) aggregation were investigated. The hydrodynamic radius ( Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1500 - 2,000,000) were calculated from their intrinsic viscosities using the Einstein viscosity relation and directly measured by quasi-elastic light scattering, and the effect of each polymer sample on RBC aggregation was studied by nephelometry and low-shear viscometry. For all three polymers, despite their different structures, samples with R-h 4 nm enhanced aggregation. Inhibition increased with R-h and was maximal at; 3 nm; above 4 nm the pro-aggregant effect increased with R-h. For comparison, the R-h of 12 plasma proteins were calculated from literature values of intrinsic viscosity or diffusion coefficient. Each protein known to promote RBC aggregation had R-h >4 nm, whereas those with R-h