Natural population dynamics and expansion of pathogenic clones of Staphylococcus aureus
Natural population dynamics and expansion of pathogenic clones of Staphylococcus aureus
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DOI：
10.1172/jci200423083
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发表时间：
2004-12-01
影响因子：
15.9
通讯作者：
van Belkum, A
中科院分区：
文献类型：
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作者：
Melles, DC;Gorkink, RFJ;van Belkum, A
使用非临床来源的大菌株采集(n=829)，确定了健康人携带的金黄色葡萄球菌的种群结构。高通量扩增片段长度多态性(AFLP)分析显示，金黄色葡萄球菌存在3个主要和2个次要的遗传簇，多位点序列分型证实了这一点。主要AFLP类群I占携带菌株的44.4%，表现出额外的异质性，而主要AFLP类群II和III有2个同源类群，占所有携带菌株的47.3%。对金黄色葡萄球菌侵袭性菌株和流行性耐甲氧西林金黄色葡萄球菌(MRSA)的联合分析表明，所有主要聚集性菌株都包含侵袭性和多重耐药菌株。然而，也发现了侵袭性分离株过多表达的簇和亚簇。例如，老年人的菌血症是由来自IVA集群的菌株比来自其他AFLP集群的菌株更频繁地引起的。此外，还检测到多耐药克隆或与皮肤病(脓疱病)相关的克隆的扩展，这表明金黄色葡萄球菌致病株存在流行潜力。此外，与携带组相比，编码潘顿-瓦伦丁杀白素的毒力基因在引起脓肿和关节炎的金黄色葡萄球菌中显著丰富。我们提供的证据表明，基本上任何人类携带的金黄色葡萄球菌基因都可以转化为威胁生命的人类病原体，但某些克隆比其他克隆的毒力更强。
The population structure of Staphylococcus aureas carried by healthy humans was determined using a large strain collection of nonclinical origin (n = 829). High-throughput amplified fragment length polymorphism (AFLP) analysis revealed 3 major and 2 minor genetic clusters of S. aureus, which were corroborated by multilocus sequence typing. Major AFLP cluster I comprised 44.4% of the carriage isolates and showed additional heterogeneity whereas major AFLP groups II and III presented 2 homogeneous clusters, including 47.3% of all carriage isolates. Coanalysis of invasive S. aureus strains and epidemic methicillin-resistant S. aureus (MRSA) revealed that all major clusters contained invasive and multiresistant isolates. However, clusters and subclusters with overrepresentation of invasive isolates were also identified. Bacteremia in elderly adults, for instance, was caused by a IVa cluster-derived strain significantly more often than by strains from other AFLP clusters. Furthermore, expansion of multiresistant clones or clones associated with skin disease (impetigo) was detected, which suggests that epidemic potential is present in pathogenic strains of S. aureus. In addition, the virulence gene encoding Panton-Valentine leukocidin was significantly enriched in S. aureus strains causing abscesses and arthritis in comparison with the carriage group. We provide evidence that essentially any S. aureus genotype carried by humans can transform into a life-threatening human pathogen but that certain clones are more virulent than others.