First passage times of driven DNA hairpin unzipping.
First passage times of driven DNA hairpin unzipping.
复制标题
驱动 DNA 发夹解链的首次通过时间。
DOI：
10.1088/1478-3975/2/3/004
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发表时间：
2005
期刊：
影响因子：
2
通讯作者：
Patey,GrenN
中科院分区：
文献类型：
--
作者：
Lakatos,Greg;Chou,Tom;Bergersen,Birger;Patey,GrenN
我们模拟电压驱动的DNA发夹通过跨膜通道的运输动力学。DNA易位过程的二维随机模型与Mathé的测量结果相吻合，Mathé通过脂质包埋的α-溶血素通道拉动自杂交的DNA发夹。由于通道太窄，不能容纳杂交的DNA，发夹的去杂交成为运输过程的限速步骤。我们表明，平均首次通过时间与电压曲线的DNA从跨膜通道的逃逸可以分为两个区域：（1）低电压区，其中的DNA滑出孔在相反的，没有经历显着的去杂交，和（2）的区域，其中的DNA去杂交的影响下施加的电压和跨膜易位。
We model the dynamics of voltage-driven transport of DNA hairpins through transmembrane channels. A two-dimensional stochastic model of the DNA translocation process is fit to the measurements of Mathé, who pulled self-hybridized DNA hairpins through lipid-embedded α-hemolysin channels. As the channel was too narrow to accommodate hybridized DNA, dehybridization of the hairpin became the rate-limiting step of the transport process. We show that the mean first passage time versus voltage curve for the escape of the DNA from the transmembrane channel can be divided into two regions:(1) a low-voltage region where the DNA slides out of the pore in reverse and without undergoing significant dehybridization, and (2) a region where the DNA dehybridizes under the influence of the applied voltage and translocates across the membrane.