A glucose biosensor with enzyme-entrapped sol–gel and an oxygen-sensitive optode membrane

A glucose biosensor with enzyme-entrapped sol–gel and an oxygen-sensitive optode membrane
复制标题

具有酶包埋溶胶-凝胶和氧敏感光极膜的葡萄糖生物传感器

DOI：
--
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
D. Xiao
D. Xiao
中科院分区：
--
文献类型：
--
作者：
X. J. Wu;Martin M. F. Choi;D. Xiao

文献摘要

被引文献

相似文献

用于连续测定的光学生物传感器 饮料中的葡萄糖基于葡萄糖氧化酶的管道化 提出了溶胶-凝胶。酶被捕获在玻璃基质内 采用溶胶-凝胶法。将基质研磨成粉末形式并 装入实验室制造的流动池中。该微型反应堆位于 连接到连续样品流系统的分光荧光计。安 氧敏感光极膜由 三(4,7-二苯基-1,10-菲咯啉)钌(II)二十二烷基 硫酸盐吸附在硅胶颗粒上并被捕获在硅橡胶中 电影。膜紧靠流动池壁放置以感测 暴露于葡萄糖后氧气含量的消耗。的变化 光极膜的发光强度可以与葡萄糖相关 浓度。温度和pH值对反应的影响 生物传感器进行了研究。储存、稳定性和重复性 生物传感器也进行了详细研究。分析范围 生物传感器的浓度为 0.06 至 30 mmol dm−3 葡萄糖， 在流动溶液中达到稳定信号所需的时间为 5–8 分钟。检测限为 6 μmol dm−3。 常见的基质干扰物如果糖、半乳糖、乳糖、棉子糖、 饮料样品中的鼠李糖、水苏糖、蔗糖等成分 没有表现出干扰。葡萄糖生物传感器已成功应用 用于测定饮料样品中的葡萄糖含量。
An optical biosensor for the continuous determination of glucose in beverages based on the canalisation of glucose oxidase into a sol–gel is presented. The enzyme was entrapped within a glass matrix by the sol–gel method. The matrix was ground to a powder form and packed into a laboratory-made flow cell. This minireactor was positioned in a spectrofluorimeter connected to a continuous sample flow system. An oxygen-sensitive optode membrane was fabricated from tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) didodecyl sulfate adsorbed on silica gel particles and entrapped in a silicone-rubber film. The membrane was situated against the wall of the flow cell to sense the depletion of oxygen content upon exposure to glucose. The change of the luminescence intensity of the optode membrane can be related to glucose concentration. The effects of temperature and pH on the response of the biosensor were investigated. Storage, stability and repeatability of the biosensor were also studied in detail. The analytical range of the biosensor was from 0.06 to 30 mmol dm−3 glucose and the time taken to reach a steady signal in a flowing solution was 5–8 min. The detection limit was found to be 6 μmol dm−3. Common matrix interferents such as fructose, galactose, lactose, raffinose, rhamnose, stachyose, sucrose and other components in beverage samples showed no interference. The glucose biosensor has been successfully applied to the determination of glucose contents of beverage samples.