Overview of Membrane Protein Purification and Crystallization
Overview of Membrane Protein Purification and Crystallization
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DOI：
10.1007/978-4-431-56030-2_6
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
T. Shimamura
中科院分区：
文献类型：
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作者：
T. Shimamura
蛋白质的三维结构为阐明蛋白质的作用机制和功能提供了重要的信息。然而，膜蛋白是难以结晶和膜蛋白的结构信息是非常有限的。这一困难主要是由于膜蛋白的疏水性和不稳定性，这增加了其纯化和结晶过程中的一些参数。近年来，一些新技术，如抗体技术和无规立方相结晶技术被应用于高质量膜蛋白晶体的生产。在这一章中，用于膜蛋白的纯化的协议，并介绍了立方晶相结晶技术。
The three-dimensional structures of proteins provide important information for elucidation of the mechanisms and functions of the proteins. However, membrane proteins are difficult to crystallize and available structural information on membrane proteins is very limited. The difficulty is mainly due to the hydrophobic nature and the instability of membrane proteins, which increase some parameters in their purification and crystallization procedures. Recently, some new techniques such as the antibody technique and the lipidic cubic phase crystallization technique were applied to the production of high-quality crystals of membrane proteins. In this chapter, the protocols for the purification of the membrane protein and the lipidic cubic phase crystallization technique are described.