The polymorphism analysis of CD169 and CD163 related with the risk of porcine reproductive and respiratory syndrome virus (PRRSV) infection
The polymorphism analysis of CD169 and CD163 related with the risk of porcine reproductive and respiratory syndrome virus (PRRSV) infection
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DOI：
10.1007/s11033-012-1857-8
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发表时间：
2012-11-01
影响因子：
2.8
通讯作者：
Zhang, S. J.
中科院分区：
文献类型：
--
作者：
Ren, Y. W.;Zhang, Y. Y.;Zhang, S. J.
猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus，PRRSV）可感染猪肺泡巨噬细胞（porcine alveolar macrophages，PAM），CD 169和CD 163是PAM表面的重要受体，但这些基因的单核苷酸多态性（single nucleotide polymorphism，SNPs）是否影响感染尚不清楚。本研究在524头猪群体中鉴定了CD 169（G1640 T、C1654 A、C4175 T）和CD 163（G2277 A、A2552 G和C2700 A）的6个SNPs，并采用两种分类方法评价了它们与PRRSV感染的关联性，以研究突变对PRRSV受体的影响。综合两种分类结果，具有CD 169-C1654 A的AA、CD 169-C4175 T的CT和CD 163-A2552 G的AA基因型的猪对PRRSV感染具有抗性。本研究结果为猪抗病育种提供了分子基础研究。但SNPs诱导的功能改变的鉴定及其分子机制尚需进一步研究。
Porcine reproductive and respiratory syndrome virus (PRRSV) could infect porcine alveolar macrophages (PAM), and the CD169 and CD163 are identified as critical receptors on the surface of PAM, but whether the single nucleotide polymorphisms (SNPs) of these genes could influence the infection is remain unclear. In this study, we identified totally 6 SNPs for CD169 (G1640T, C1654A, C4175T) and CD163 (G2277A, A2552G and C2700A), and evaluated their associations with PRRSV infection using two classified methods in a 524 pig population to investigate the effects of mutations on the PRRSV receptors. The pigs with genotypes of AA of CD169-C1654A, CT of CD169-C4175T and AA of CD163-A2552G appeared to resistant to the PRRSV infection by the combination of two classified results. The results provided fundamental molecular investigation to promote pig breeding with disease resistance. However, the identification of functional changes induced by SNPs and molecular mechanism were need further research.