Deposition of complement protein C3b on mixed self-assembled monolayers carrying surface hydroxyl and methyl groups studied by surface plasmon resonance
Deposition of complement protein C3b on mixed self-assembled monolayers carrying surface hydroxyl and methyl groups studied by surface plasmon resonance
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DOI：
10.1002/jbm.a.10067
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发表时间：
2003-09-01
影响因子：
4.9
通讯作者：
Iwata, H
中科院分区：
文献类型：
--
作者：
Hirata, I;Hioko, Y;Iwata, H
由于补体激活被认为是人工医疗器械应用于患者时宿主防御系统的共同反应，补体系统与人工材料相互作用的研究已经投入了大量的精力。然而，一些不确定性仍然存在，部分原因是缺乏表征良好的表面和合适的分析方法来研究生理条件下人造材料上发生的表面现象。在这项研究中，我们采用自组装单层（SAMs）和表面等离子体共振（SPR）技术来研究血清补体与表征良好的表面的相互作用。用11-巯基-1-十一醇（C11-OH）和正壬硫醇、正十二硫醇和正十六硫醇中的一种制备了携带不同浓度羟基的自组装单层膜。随着SAMs中C11-OH含量的增加，SAMs上的NHS沉积量增加，在NHS沉积层上形成的抗c3b抗体固定化量也随着SAMs中C11-OH含量的增加而增加。这些结果清楚地表明，通过激活补体系统产生的大量C3b与富含羟基的表面共价结合并被其吸附。SAMs和SPR技术的结合适用于补体系统与固体表面相互作用的研究，其结果将为合理设计合成材料的生物相容性表面提供基础信息。
Since complement activation is recognized as a common response of the host defense system when an artificial medical device is applied to a patient, great effort has been devoted to studies on the interaction of the complement system with artificial materials. However, some uncertainties remain, partially because of the lack of well characterized surfaces and suitable analytic methods for study of the surface phenomena that occur on artificial materials under physiologic conditions. In this study, we employed self-assembled monolayers (SAMs) and the surface plasmon resonance (SPR) technique to study interactions of the serum complement with well characterized surfaces. Self-assembled monolayers carrying various concentrations of hydroxyl groups were prepared using 11-mercapto-1-undecanol (C11-OH) and one of n-nonanethiol, n-dodecanethiol, and n-hexadecanethiol. The amount of NHS deposition on the SAMs increased with increasing C11-OH content of the SAMs, and the amount of anti-C3b antibody immobilization formed on the NHS deposition layers increased with increasing C11-OH content of the SAMs. These results clearly demonstrate that a large amount of C3b, produced through the activation of the complement system, binds covalently to and is adsorbed by hydroxyl-group-rich surfaces. The combination of SAMs and the SPR technique is suitable for studying the interaction of the complement system with solid surfaces, and the results should give basic information needed for a rational design of biocompatible surfaces on synthetic materials.