“Swiss roll” -like bioactive hybrid scaffolds for promoting bone tissue ingrowth and tendon-bone healing after anterior cruciate ligament reconstruction
“Swiss roll” -like bioactive hybrid scaffolds for promoting bone tissue ingrowth and tendon-bone healing after anterior cruciate ligament reconstruction
复制标题
瑞士卷式生物活性混合支架，用于促进前十字韧带重建后骨组织向内生长和腱骨愈合
DOI：
10.1039/c9bm01703h
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发表时间：
2020
影响因子：
6.6
通讯作者：
Shiyi Chen
中科院分区：
文献类型：
--
作者：
Peng Zhang;Fei Han;Tianwu Chen;Ziying Wu;Shiyi Chen
前交叉韧带（ACL）重建术中移植物的选择是运动医学中的一个关键问题。以往的研究表明，由单一材料制备的支架不能实现移植物和自体骨隧道之间的完全整合。为了解决这个问题，我们假设，结合可降解支架与不可降解支架可以产生一种新的混合韧带的优点，这两种类型的支架。在这项研究中，骨形态发生蛋白7（BMP-7）加载的聚己内酯（PCL）纳米纤维膜首先制造的混合韧带的可降解部分，通过使用逐层（LbL）自组装。然后，我们通过将这种纳米纤维膜和聚对苯二甲酸乙二醇酯（PET）网状织物（不可降解部分）卷起成“瑞士卷”结构，制造了一种多功能的新型混合韧带。体外实验结果表明，该复合韧带能显著提高纯PET韧带的生物相容性，并进一步促进细胞矿化。体内实验结果表明，这种独特的结构显著促进了混合韧带与骨隧道的整合，从而实现ACL重建手术后的真实的“韧带化”。这些结果表明，这种新型的混合仿生人工韧带支架为ACL重建的移植物选择提供了新的方向。
The choice of grafts for anterior cruciate ligament (ACL) reconstruction is a critical issue in sports medicine. Previous studies have revealed that scaffolds prepared from a single material could not achieve complete integration between the graft and autogenous bone tunnel. To solve this problem, we hypothesize that combining degradable scaffolds with nondegradable scaffolds can produce a novel hybrid ligament with the advantages of both types of scaffolds. In this study, a bone morphogenetic protein 7 (BMP-7)-loaded polycaprolactone (PCL) nanofibrous membrane was first manufactured as the degradable part of the hybrid ligament by using layer-by-layer (LbL) self-assembly. Then, we fabricated a multifunctional novel hybrid ligament by rolling up this nanofibrous membrane and polyethylene terephthalate (PET) mesh fabric (nondegradable part) into a “swiss roll” structure. The in vitro experimental results showed that this hybrid ligament could significantly improve the biocompatibility of pure PET ligament and further promote cell mineralization. The in vivo experimental results showed that this unique structure significantly promoted the integration of hybrid ligaments and bone tunnels, thereby achieving real “ligamentization” after ACL reconstruction surgery. These results suggest that this novel hybrid biomimetic artificial ligament scaffold provides a new direction for graft selection for ACL reconstruction.