Time-specific effects of acute eccentric exercise on myostatin, follistatin and decorin in the circulation and skeletal muscle in rats.
Time-specific effects of acute eccentric exercise on myostatin, follistatin and decorin in the circulation and skeletal muscle in rats.
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DOI：
10.33549/physiolres.934833
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发表时间：
2022-10
影响因子：
2.1
通讯作者：
X. Liu;N. Zhang;B. Sun;B. Wang
中科院分区：
文献类型：
--
作者：
X. Liu;N. Zhang;B. Sun;B. Wang
肌生成抑制素（Myostatin，MSTs）是骨骼肌重要的负性调节因子，在骨骼肌健康中起重要作用。在以往的研究中，我们发现在骨骼肌损伤修复过程中，Mc的表达是不同的。因此，我们探讨了离心运动后骨骼肌损伤修复过程中不同时间点MMP3的表达变化。此外，MclO在体内受卵泡抑素（FST）和核心蛋白聚糖（DCN）的调节，因此本研究检测了离心运动后骨骼肌损伤修复过程中FST、DCN和MclO在循环和骨骼肌中的时间特异性变化，探讨运动性肌肉损伤修复过程中MclO变化的原因，为促进肌肉损伤修复提供依据。大鼠进行一次性离心运动。分别于运动后即刻（D0）、运动后1d（D1）、2d（D2）、3d（D3）、7d（W1）和14d（W2）采集血液和骨骼肌（n=8）。检测血清中MRF、FST、DCN的含量及肌肉中MRF、FST、DCN mRNA和蛋白的表达。除运动后即刻外，血液中MMP2的变化与DCN和FST的变化呈相反趋势。腓肠肌DCN和Mexo mRNA和蛋白的变化趋势不一致，Mexo和DCN在腓肠肌中存在转录后调控。急性离心运动可刺激DCN和FST分泌入循环，抑制MRF。FST和DCN可调节急性离心运动后的运动负荷。
Myostatin (MSTN), an important negative regulator of skeletal muscle, plays an important role in skeletal muscle health. In previous study, we found that the expression of MSTN was different during skeletal muscle injury repair. Therefore, we explored the expression changes of MSTN at different time points during skeletal muscle injury repair after eccentric exercise. In addition, MSTN is regulated by follistatin (FST) and decorin (DCN) in vivo, so our study examined the time-specific changes of FST, DCN and MSTN in the circulation and skeletal muscle during skeletal muscle injury repair after eccentric exercise, and to explore the reasons for the changes of MSTN in the process of exercise-induced muscle injury repair, to provide a basis for promoting muscle injury repair. The rats performed one-time eccentric exercise. Blood and skeletal muscle were collected at the corresponding time points, respectively immediate after exercise (D0), one day (D1), two days (D2), three days (D3), seven days (W1) and fourteen days (W2) after exercise (n=8). The levels of MSTN, FST, DCN in serum and mRNA and protein expression in muscle were detected. MSTN changes in the blood and changes in DCN and FST showed the opposite trend, except immediately after exercise. The change trends of mRNA and protein of gastrocnemius DCN and MSTN are inconsistent, there is post-transcriptional regulation of MSTN and DCN in gastrocnemius. Acute eccentric exercise might stimulate the secretion of DCN and FST into the circulation and inhibit MSTN. MSTN may be regulated by FST and DCN after acute eccentric exercise.