Difference in glucose intolerance between C57BL/6J and ICR strain mice with streptozotocin/nicotinamide-induced diabetes
Difference in glucose intolerance between C57BL/6J and ICR strain mice with streptozotocin/nicotinamide-induced diabetes
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DOI：
10.2220/biomedres.33.63
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发表时间：
2012-02-01
影响因子：
1.2
通讯作者：
Ueno, Hitoshi
中科院分区：
文献类型：
--
作者：
Shimizu, Ryo;Sakazaki, Fumitoshi;Ueno, Hitoshi
比较了烟酰胺（NA）和链脲佐菌素（STZ）诱导的C57 BL/6 J和ICR品系小鼠糖尿病模型的血糖和血浆胰岛素水平，以建立一种适合的实验性糖尿病小鼠模型。注射NA（120 mg/kg）后15 min，每隔1 d腹腔注射STZ（100 mg/kg）2次，每周监测非空腹血糖水平，共5周。ICR小鼠的血糖水平逐渐升高，并且在观察结束时比C57 BL/6 J小鼠高约2倍。ICR小鼠血浆胰岛素水平较C57 BL/6 J小鼠低。ICR小鼠在口服葡萄糖耐量试验中也表现出明显的葡萄糖不耐受。这些结果表明，ICR品系比C57 BL/6 J品系作为NA/STZ诱导的轻度糖尿病小鼠模型更敏感。
Blood glucose and plasma insulin levels between C57BL/6J and ICR strain mice with nicotinamide (NA) and streptozotocin (STZ)-induced diabetes were compared to establish a suitable strain of the experimental diabetic mouse model. The mice were intraperitoneally treated twice with STZ (100 mg/kg) 15 min after injection of NA (120 mg/kg) at a 1-day interval, and non-fasting blood glucose level was then weekly monitored for 5 weeks. The blood glucose level in ICR mice gradually increased and was about 2-times higher than that in C57BL/6J mice at the end of the observation. The plasma insulin level in ICR mice was comparatively low, compared with that in C57BL/6J mice. ICR mice were also markedly glucose-intolerant when oral glucose tolerance test was performed. These results indicate that ICR strain is more sensitive than C57BL/6J strain as a mouse model with NA/STZ-induced mild diabetes.