Rooster testicular germ cells and epididymal sperm contain P450 aromatase.
Rooster testicular germ cells and epididymal sperm contain P450 aromatase.
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公鸡睾丸生殖细胞和附睾精子中含有P450芳香酶。
DOI：
10.1095/biolreprod53.6.1259
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发表时间：
1995
影响因子：
3.6
通讯作者：
Bahr,JM
中科院分区：
文献类型：
--
作者：
Kwon,S;Hess,RA;Bunick,D;Nitta,H;Janulis,L;Osawa,Y;Bahr,JM
我们最近发现细胞色素P450芳香化酶（P450 arom）存在于哺乳动物睾丸的生殖细胞中，并且能够在雄性生殖道中将雄激素转化为雌激素。本研究的目的是确定是否睾丸生殖细胞和附睾精子的鸟类物种也能够合成雌激素。使用针对纯化的人胎盘细胞色素P450 arom产生的抗血清，通过免疫细胞化学方法将P450 arom定位于公鸡睾丸和附睾区域。粗线期精母细胞、圆形精子细胞、细长精子细胞、晚期精子细胞的鞭毛和附睾区的精子中存在免疫染色。附睾区无纤毛细胞也呈阳性反应。然而，附睾区P450 aromin的mRNA的缺乏表明，免疫反应蛋白存在于附睾区是不是在这个区域合成。用~ 3 H_（20）法测定附睾精子中的P450免疫反应活性。这些数据的基础上，我们得出结论，公鸡睾丸生殖细胞和附睾精子的雌激素，在睾丸和附睾区的上皮细胞的鸟类物种的生殖上皮细胞的潜在调节剂或调制器的合成的网站。
We recently found that cytochrome P450 aromatase (P450arom) is present in germ cells of the mammalian testis and is capable of converting androgens to estrogens in the male reproductive tract. The objective of the present study was to determine whether testicular germ cells and epididymal sperm of an avian species are also capable of synthesizing estrogen. P450aromwas localized in the roostertestis and epididymal region by immunocytochemistry, using an antiserum generated against purified human placental cytochrome P450arom. Immunostaining was present in pachytene spermatocytes, round spermatids, elongated spermatids, flagella of late spermatids, and sperm in the epididymal region. A positive reaction was also found in nonciliated cells of the epididymal region. However, the absence of mRNA for P450aromin the epididymal region indicated that the immunoreactive protein present in the epididymal region is not synthesized in this region. The immunoreactive P450aromfound in epididymal sperm was shown to be active through use of a3H20 assay. On the basis of these data, we conclude that rooster testicular germ cells and epididymal sperm are sites for the synthesis of estrogen, a potential regulator or modulator of germinal epithelium in the testis and the epithelium of the epididymal region of the avian species.