The entire nucleotide sequence and classification of a hepatitis C virus isolate of a novel genotype from an Indonesian patient with chronic liver disease.
The entire nucleotide sequence and classification of a hepatitis C virus isolate of a novel genotype from an Indonesian patient with chronic liver disease.
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来自印尼慢性肝病患者的新型基因型丙型肝炎病毒分离株的完整核苷酸序列和分类。
DOI：
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发表时间：
1994
影响因子：
3.8
通讯作者：
M. Mayumi
中科院分区：
文献类型：
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作者：
H. Okamoto;M. Kojima;M. Sakamoto;H. Iizuka;S. Hadiwandowo;Sumoharjo Suwignyo;Y. Miyakawa;M. Mayumi
从印度尼西亚的慢性肝病患者中获得了三种丙型肝炎病毒（HCV）分离株，这些患者无法分类为先前报道的任何基因型I/1a，II/1b，III/2a，IV/2b或V/3a。确定了一个HCV分离株（HC-G9）的完整核苷酸序列;基于其跨越2927个核苷酸（nt）的部分序列内> 95%的相似性，其余两个分离株具有相同的基因型。HC-G9基因组全长9440 nt，其中5'端非翻译区341 nt，开放阅读框9033 nt，编码3011个氨基酸的多聚蛋白，3'端非翻译区66 nt（不包括3'端17 ~ 47 nt的U片段）。其核苷酸序列与已知全长序列的基因型I/1a至IV/2b的14个HCV基因组中的任何一个的核苷酸序列相差20%至33%。采用算术平均值的未加权配对组法，HC-G9位于HCV基因型I/1a和II/1b所属的系统发育树的一个主要分支（第1组）。因此，建议将HC-G9的新基因型称为1c。开发了一种方法，以确定基因型1c的PCR与引物推导出的核心基因，是特定的it. Since基因型1c检测到7（15%）的48例慢性肝病患者的HCV RNA样本从印度尼西亚，但没有在任何1097从世界其他地区，它似乎已经演变，并留在印度尼西亚。除了其流行病学的重要性，基因型1c HCV与肝脏疾病的严重程度及其对干扰素的反应的关联值得评估。
Three hepatitis C virus (HCV) isolates were obtained from patients with chronic liver diseases in Indonesia which were not classifiable into any of the genotypes I/1a, II/1b, III/2a, IV/2b or V/3a reported previously. The entire nucleotide sequence was determined for one HCV isolate (HC-G9); the remaining two isolates were of the same genotype based on a > 95% similarity within their partial sequences spanning 2927 nucleotides (nt). The HC-G9 genome consisted of 9440 nt including the 5' untranslated region of 341 nt, an open reading frame of 9033 nt coding for a polyprotein of 3011 amino acids and the 3' untranslated region of 66 nt (U stretch of 17 to 47 nt at the extreme 3' terminus excluded). It differed by 20 to 33% in nucleotide sequence from any of 14 HCV genomes of genotypes I/1a to IV/2b whose full-length sequences are known. By the unweighted pair-group method with arithmetic mean, HC-G9 was on a major branch (group 1) of the phylogenetic tree of HCV to which genotypes I/1a and II/1b belong. It is proposed, therefore, that the novel genotype for HC-G9 should be called 1c. A method was developed to identify genotype 1c by PCR with a primer deduced from the core gene that was specific to it. Since genotype 1c was detected in seven (15%) of 48 HCV RNA samples from Indonesian patients with chronic liver disease, but not in any of 1097 from other districts of the world, it appears to have evolved and remained in Indonesia. In addition to its epidemiological importance, the association of genotype 1c HCV with the severity of liver disease and its response to interferons deserve to be evaluated.