Development of ic-ELISAs for the detection of Bisphenol A diglycidyl ether (BADGE) and Its Derivatives in Canned Luncheon Meats
Development of ic-ELISAs for the detection of Bisphenol A diglycidyl ether (BADGE) and Its Derivatives in Canned Luncheon Meats
复制标题
开发 ic-ELISA 检测罐头午餐肉中的双酚 A 二缩水甘油醚 (BADGE) 及其衍生物
DOI：
10.1021/acsfoodscitech.1c00393
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Zhen Zhang
中科院分区：
文献类型：
--
作者：
Can Zhang;Jinnuo Hu;Jingya Song;Maotong Wu;Zhen Zhang
罐头食品因其方便、卫生、营养而被广泛消费。双酚A二缩水甘油醚（BADGE）是环氧氯丙烷和双酚A的工业产品，广泛存在于罐头食品的内涂层中。 BADGE在储存过程中可发生水解或氯化反应，形成双酚A(2,3-二羟丙基)缩水甘油醚(BADGE·H2O)、双酚A(3-氯-2-羟丙基)缩水甘油醚(BADGE·HCl)、双酚A(3-氯-2-羟丙基)(2,3-二羟丙基)缩水甘油醚等多种衍生物。 (BADGE·HCl·H2O)，严重威胁消费者的健康。本研究成功合成了两种半抗原，并通过新西兰兔皮下免疫获得抗体。建立间接竞争酶联免疫吸附试验（ic-ELISA）检测BADGE、BADGE·H2O、BADGE·HCl和BADGE·HCl·H2O，测定其IC15值（抑制率15%时的浓度）分别为0.73、0.39、0.78和1.45 ng/mL。罐装午餐肉样品中不同添加水平的 BADGE 及其衍生物的回收率为 76.70% 至 98.35%。所提出的 ic-ELISA 检测的结果也通过 HPLC 分析进行了验证，显示出良好的一致性（R2 = 0.9861）。
Canned foods are widely consumed because of their convenience, hygiene, and nutrition. Bisphenol A diglycidyl ether (BADGE), an industrial product of epichlorohydrin and bisphenol A, widely exists in the inner coating of canned foods. A hydrolysis or chlorination reaction of BADGE can occur in the process of storage, forming a variety of derivatives such as bisphenol A (2,3-dihydroxypropyl) glycidyl ether (BADGE·H2O), bisphenol A (3-chloro-2-hydroxypropyl) glycidyl ether (BADGE·HCl), and bisphenol A (3-chloro-2-hydroxypropyl) (2,3-dihydroxypropyl) glycidyl ether (BADGE·HCl·H2O), which seriously threaten consumer’s health. In this research, two haptens were successfully synthesized, and antibodies were obtained by the subcutaneous immunization of New Zealand rabbits. Indirect competitive enzyme-linked immunosorbent assays (ic-ELISAs) were established for the detection of BADGE, BADGE·H2O, BADGE·HCl, and BADGE·HCl·H2O, and the IC15values (concentrations at an inhibition rate of 15%) were determined as 0.73, 0.39, 0.78, and 1.45 ng/mL, respectively. The recovery of BADGE and its derivatives at various spiking levels in canned luncheon meat samples ranged from 76.70 to 98.35%. The results detected by the proposed ic-ELISAs were also validated by HPLC analysis, which showed a good agreement (R2= 0.9861).