Cellular responses to mechanical loading in vitro
Cellular responses to mechanical loading in vitro
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DOI：
10.1002/jbmr.5650050905
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发表时间：
1990-09
影响因子：
6.2
通讯作者：
A. Haj;Sarah L. Minter;S. Rawlinson;R. Suswillo;L. Lanyon
中科院分区：
文献类型：
--
作者：
A. Haj;Sarah L. Minter;S. Rawlinson;R. Suswillo;L. Lanyon
已经建立了一种技术，其中松质骨活检可以同时灌注并承受机械载荷。基于cAMP对甲状旁腺激素的反应、细胞内乳酸脱氢酶活性和电子显微镜形态，对24小时内的细胞活力进行评估。两个细胞对机械负荷的反应被证明类似于体内“成骨”负荷，如前所述。这些是（1）在加载后立即在衬里细胞中的细胞内G6PD升高，和（2）在加载后6小时在骨细胞中测量的RNA合成增加。在体内，成骨反应负荷调制消炎痛。在这些体外实验中，吲哚美辛的加入抑制了负荷相关的G6PD和RNA反应。
A technique has been established in which cancellous bone biopsies may be simultaneously perfused and subjected to mechanical load bearing. Assessments of cell viability over a period of 24 h were based on the cAMP response to parathyroid hormone, intracellular lactate dehydrogenase activity, and electron micrograph morphology. Two cellular responses to mechanical loading were demonstrated similar to those that follow “osteogenic” loading in vivo, as reported previously. These were (1) a rise in intracellular G6PD in lining cells immediately after loading, and (2) an increase in RNA synthesis measured in osteocytes 6 h after loading. In vivo the osteogenic response to loading was modulated by indomethacin. In these in vitro experiments, addition of indomethacin inhibited both the loading‐related G6PD and the RNA responses.