Analysis of invasion-metastasis in pancreatic cancer: Correlation between the expression and arrangement of tight junction protein-2 and cell dissociation in pancreatic cancer cells.
Analysis of invasion-metastasis in pancreatic cancer: Correlation between the expression and arrangement of tight junction protein-2 and cell dissociation in pancreatic cancer cells.
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DOI：
10.3892/mmr_00000232
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发表时间：
2009-11
影响因子：
3.4
通讯作者：
Lei Zhou;X. Tan;Wei Wang;Baosheng Wang;X. Dai;Jingang Liu
中科院分区：
文献类型：
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作者：
Lei Zhou;X. Tan;Wei Wang;Baosheng Wang;X. Dai;Jingang Liu
高侵袭和高转移率是胰腺癌的重要特征之一。我们最近的研究通过cDNA微阵列分析，发现紧密连接蛋白-2（Tjp-2）在高（PC-1.0）和弱（PC-1）侵袭转移性胰腺癌细胞中是一个与侵袭转移相关的差异表达基因。紧密连接的结构和功能的变化与癌的发生和肿瘤的发展相关。本研究采用RT-PCR、Western blotting和免疫细胞化学等方法研究胰腺癌组织中Tjp-2的表达和定位与细胞解离的关系。Tjp-2 mRNA和蛋白在PC-1.0和PC-1细胞中表达差异。此外，解离因子（DF）或U 0126（MEK抑制剂）的加入显着诱导Tjp-2的mRNA表达和蛋白质细胞内定位的变化，并在PC-1.0和PC-1细胞的同时细胞解离。然而，DF或U 0126处理不影响Tjp-2的蛋白表达。目前的结果表明，Tjp-2是通过基因表达和细胞内定位的变化参与胰腺癌细胞的细胞解离的调节。Tjp-2可能成为胰腺癌侵袭转移分子治疗的新靶点。
High frequency of invasion and metastasis is one of the key characteristics of pancreatic cancer. In our recent study, tight junction protein-2 (Tjp-2) was identified as a differentially expressed gene related to invasion-metastasis in highly (PC-1.0) and weakly (PC-1) invasive and metastatic pancreatic cancer cells by cDNA microarray analysis. Changes in the structure and function of tight junctions are correlated with carcinogenesis and tumour development. In this study, RT-PCR, Western blotting and immunocytochemistry were used to study the correlation between the expression and localisation of Tjp-2 and cell dissociation in pancreatic cancer. Tjp-2 mRNA and protein were differentially expressed in PC-1.0 and PC-1 cells. Furthermore, the addition of dissociation factor (DF) or U0126 (a MEK inhibitor) significantly induced changes in the mRNA expression and protein intracellular localisation of Tjp-2, and in the simultaneous cell dissociation of PC-1.0 and PC-1 cells. However, protein expression of Tjp-2 was not affected by DF or U0126 treatment. The current results indicate that Tjp-2 is involved in the regulation of cell dissociation in pancreatic cancer cells through changes in gene expression and intracellular localisation. Tjp-2 may serve as a new target for molecular therapies that prevent the invasion and metastasis of pancreatic cancer.