Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
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DOI：
10.1590/s0102-695x2011005000080
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发表时间：
2011-04-01
期刊：
影响因子：
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通讯作者：
Noseda, Miguel D.
中科院分区：
文献类型：
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作者：
Ducatti, Diogo R. B.;Colodi, Franciely G.;Noseda, Miguel D.
通过对商品琼脂糖和卡帕-卡拉胶进行部分酸水解制备了琼脂糖和卡拉胶低聚糖。通过凝胶过滤层析纯化二糖和四糖，并通过核磁共振（1D和2D）光谱和ESIMS进行表征。得到的低聚糖有：琼脂糖、琼脂四糖、卡帕-卡拉糖和卡帕-卡拉糖。以琼脂糖和琼脂四糖为标准，建立了高效阻垢色谱法（HPSEC），用于研究琼脂糖和低聚糖的水解率。0.1 M TFA， 65℃水解6小时，主要产生二糖和四糖。本研究所建立的低聚糖生产和评价方法在生产具有生物活性的低聚糖方面具有良好的潜力。
Agaro- and carra-oligosaccharides were produced by partial acid hydrolysis of commercial agarose and kappa-carrageenan. Di- and tetrasaccharides were purified by gel filtration chromatography and characterized by NMR (1D and 2D) spectroscopy and ESIMS. The following oligosaccharides were obtained: agarobiose, agarotetraose, kappa-carrabiose and kappa-carratetraose. Agarobiose and agarotetraose were used as standards to develop a high performance size exclusion chromatography (HPSEC) method which was utilized to study the hydrolysis rate of agarose and oligosaccharide production. Six hours of hydrolysis (0.1 M TFA, 65 degrees C) produced mainly di- and tetrasaccharides. The methodology for oligosaccharide production and evaluation developed in the present work shows good potential for the production of bioactive oligosaccharides.