Arabidopsis AtMPV17, a homolog of mice MPV17, enhances osmotic stress tolerance
Arabidopsis AtMPV17, a homolog of mice MPV17, enhances osmotic stress tolerance
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DOI：
10.1007/s12298-020-00834-x
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发表时间：
2020-06
影响因子：
3.5
通讯作者：
Jiwoong Wi;Yeonju Na;E. Yang;Jung-Hyun Lee;W. Jeong;D. Choi
中科院分区：
文献类型：
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作者：
Jiwoong Wi;Yeonju Na;E. Yang;Jung-Hyun Lee;W. Jeong;D. Choi
人MPV17基因或酵母同源基因SYM1的突变导致线粒体DNA枯竭。MPV17同源基因也存在于包括拟南芥在内的植物中，但这些基因的功能尚不清楚。其中，AtMPV17蛋白以MPV17和SYM1的形式定位于线粒体。酵母sym1基因敲除突变体不能在37℃含乙醇的培养基上生长。AtMPV17补充了37℃下sym1Yed MPV17同源细胞的乙醇生长缺陷，表明AtMPV17是SYM1的功能同源基因。但在萌发和幼苗生长过程中，atmpv17突变体对ABA和甘露醇的敏感性高于野生型植株。Atmpv17基因敲除突变体在含有ABA和甘露醇的培养基上的生长抑制被AtMPV17过表达所补充。这些结果表明，AtMPV17与植物的渗透胁迫耐受性有关。
Mutation in the humanMPV17gene or the functional yeast orthologueSYM1result in mitochondrial DNA depletion.MPV17homologs are also found in plants includingArabidopsis, but the function of these genes remain unclear.Arabidopsisgenome contains 10MPV17homologs. Among these, the AtMPV17 protein was localized in mitochondria as MPV17 and SYM1. The yeastsym1knock out mutant cannot grow on ethanol-containing medium at 37 °C.AtMPV17complements the ethanol growth defection ofsym1yeastMPV17ortholog cells at 37 °C, suggesting thatAtMPV17is a functional ortholog ofSYM1.AtMPV17knock out mutant,atmpv17show similar growth and seed development to those of the wild-type plant on normal growth condition. However,atmpv17mutant is more sensitive to ABA and mannitol during germination and seedling growth than wild type plants. Growth retardation of theatmpv17knock out mutant on medium containing ABA and mannitol is complemented byAtMPV17overexpression. These results suggest that theAtMPV17contributes to osmotic stress tolerance in plants.