Transcriptional regulators involved in responses to volatile organic compounds in plants
Transcriptional regulators involved in responses to volatile organic compounds in plants
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参与植物挥发性有机化合物反应的转录调节因子
DOI：
10.1096/fasebj.2021.35.s1.00109
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Kazushige Touhara
中科院分区：
文献类型：
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作者：
Ayumi Nagashima‐KASAHARA;Takumi Higaki;Takao Koeduka;Ken Ishigami;Satoko Hosokawa;Hidenori Watanabe;Kenji Matsui;Seiichiro Hasezawa;Kazushige Touhara
据报道，自20世纪80年代以来，在田间与食草动物侵染的植物相邻的植物获得了更高水平的抗损害性。目前的理解是，这是由于受伤植物释放的挥发性有机化合物（VOC）引发邻近健康植物的防御反应。然而，人们对植物中检测VOCs的分子基础知之甚少。在这里，我们报告了一种转录调控蛋白的鉴定，该蛋白具有VOC结合活性，并参与烟草中的VOC传感。通过筛选烟草BY-2细胞和烟草植株中诱导胁迫应答基因表达的挥发物，我们发现一些倍半萜类化合物，即香芹烯类似物，诱导了胁迫应答基因的表达。这些结果提供了证据表明，植物感知分子种类的挥发性有机化合物，并激励我们专注于挥发性有机化合物结合分子来分析挥发性有机化合物传感的机制。因此，我们鉴定了TOPLESS样蛋白（TPLs），转录共阻遏物，其特异性结合于卡顺烯衍生物连接的珠。重要的是，当TPLs在BY-2细胞系中过表达时，由卡连菜烯引起的应激反应基因表达减少。这些结果表明，TPLs和香芹烯类似物的相互作用导致的目的基因的诱导，从而阐明了在植物中的VOC传感机制。
It has been reported since the 1980s that plants neighboring to herbivore‐infested plants in field acquire higher levels of resistance to damages. The current understanding is that it is due to volatile organic compounds (VOCs), released from wounded plants, which trigger defensive responses in neighboring healthy plants. The molecular basis for sensing VOCs in plants, however, is poorly understood. Here, we report the identification of a transcriptional regulatory protein that has a VOC‐binding activity and is involved in VOC sensing in tobacco. Via screening for volatiles that induce stress‐responsive gene expression in tobacco BY‐2 cell and tobacco plants, we found that some sesquiterpenes, caryophyllene analogs, induced stress‐responsive gene. These results provide evidence that plants sense molecular species of VOCs, and motivated us focusing on VOC binding molecules to analyze the mechanisms underlying volatile sensing. As a result, we identified TOPLESS‐like proteins (TPLs), transcriptional co‐repressors, that were specifically bound to the caryophyllene derivative‐linked beads. Importantly, when TPLs was overexpressed in BY‐2 cell line, stress‐responsive gene expression by the caryophyllene was reduced. These results suggest that the interaction of TPLs and caryophyllene analogs lead to the induction of target genes and thus shed light on the mechanism of VOC sensing in plants.