Micro-Optical Sectioning Tomography to Obtain a High-Resolution Atlas of the Mouse Brain
Micro-Optical Sectioning Tomography to Obtain a High-Resolution Atlas of the Mouse Brain
复制标题
微光学断层扫描获得小鼠大脑的高分辨率图谱
DOI：
10.1126/science.1191776
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发表时间：
2010-12-03
期刊：
影响因子：
56.9
通讯作者：
Luo, Qingming
中科院分区：
文献类型：
--
作者：
Li, Anan;Gong, Hui;Luo, Qingming
更好的大脑地图一个完整的神经元连接在整个大脑的高分辨率图集应该从根本上推进我们对动物神经系统的组织和功能的理解。现在，A。李等人（p.1404，11月4日在线发表）描述了一种自动化系统，显微光学切片断层摄影术，其允许在完整的小鼠脑中对神经元的形态和空间位置以及神经突的痕迹进行三维绘图。已经开发了以1微米分辨率采集整个小鼠大脑的光显微镜数据。神经解剖结构被认为是理解脑功能和功能障碍的基础。然而，现有的成像工具在中尺度水平上对神经回路的全脑映射具有局限性。我们开发了一种显微光学断层扫描（MOST）系统，可以提供厘米大小的整个小鼠大脑的微米级断层扫描。使用MOST，我们获得了高尔基染色的整个小鼠脑的神经突水平的三维结构数据集。神经元的形态、空间位置和突起的痕迹可以清楚地区分。我们发现相邻的浦肯野细胞相互粘附。
Better Brain Maps A high-resolution atlas of the complete neuronal connectivity in a whole brain should fundamentally advance our understanding of the organization and function of animal nervous systems. Now, A. Li. et al. (p. 1404, published online 4 November) describe an automated system, micro-optical sectioning tomography, that allowed three-dimensional mapping of the morphology and spatial location of neurons and traces of neurites in a whole, intact mouse brain. Acquisition of light microscopic data at 1-micrometer resolution for an entire mouse brain has been developed. The neuroanatomical architecture is considered to be the basis for understanding brain function and dysfunction. However, existing imaging tools have limitations for brainwide mapping of neural circuits at a mesoscale level. We developed a micro-optical sectioning tomography (MOST) system that can provide micrometer-scale tomography of a centimeter-sized whole mouse brain. Using MOST, we obtained a three-dimensional structural data set of a Golgi-stained whole mouse brain at the neurite level. The morphology and spatial locations of neurons and traces of neurites could be clearly distinguished. We found that neighboring Purkinje cells stick to each other.