Endothelial cell scaffolds generated by 3D direct writing of biodegradable polymer microfibers
Endothelial cell scaffolds generated by 3D direct writing of biodegradable polymer microfibers
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DOI：
10.1016/j.biomaterials.2010.11.023
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发表时间：
2011-03-01
期刊：
影响因子：
14
通讯作者：
Keynton, Robert S.
中科院分区：
文献类型：
--
作者：
Berry, Scott M.;Warren, Sean P.;Keynton, Robert S.
大型（厚度> 100μm）组织的工程需要微血管网络来供应营养并清除废物。为了在体外产生微血管系统，需要支架来机械支撑和刺激内皮细胞（EC）粘附和生长。目前，EC 支架是通过将聚合物或其他生物材料图案化为通常具有各向同性形态的结构来生产的，例如多孔膜和纤维垫。我们提出了一种新的“直写”工艺，用于制造由悬浮聚合物微纤维组成的支架，这些微纤维在 3D 中精确定向，为选择性引导细胞沿所需路径生长提供定向结构。通过调节系统参数，可预测且可重复地控制用该工艺生产的纤维的直径，从而能够使用各种可生物降解的聚合物生产具有微血管级直径（5-20μm）的纤维。这些支架成功地接种了 EC，EC 符合纤维的几何形状，并在一周内增殖。 (C) 2010 Elsevier Ltd. 保留所有权利。
The engineering of large (thickness > 100 mu m) tissues requires a microvascular network to supply nutrients and remove waste. To produce microvasculature in vitro, a scaffold is required to mechanically support and stimulate endothelial cell (EC) adhesion and growth. Scaffolds for ECs are currently produced by patterning polymers or other biomaterials into configurations which often possess isotropic morphologies such as porous films and fibrous mats. We propose a new "direct-write" process for fabricating scaffolds composed of suspended polymer microfibers that are precisely oriented in 3D, providing directional architecture for selectively guiding cell growth along a desired pathway. The diameters of the fibers produced with this process were predictably and repeatably controlled through modulation of the system parameters, enabling production of fibers with microvascular-scale diameters (5-20 mu m) from a variety of biodegradable polymers. These scaffolds were successfully seeded with ECs, which conformed to the geometry of the fibers and proliferated over the course of one week. (C) 2010 Elsevier Ltd. All rights reserved.