A new murine model of autoimmune orchids induced by immunization with viable syngeneic testicular germ cells alone. I. Immunological and histological studies
A new murine model of autoimmune orchids induced by immunization with viable syngeneic testicular germ cells alone. I. Immunological and histological studies
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一种新的自身免疫兰花小鼠模型，通过单独使用活的同基因睾丸生殖细胞进行免疫诱导。
DOI：
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发表时间：
1991
影响因子：
4.6
通讯作者：
K. Hojo
中科院分区：
文献类型：
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作者：
M. Itoh;C. Hiramine;K. Hojo
实验性自身免疫性睾丸炎（EAO）在C3H/He小鼠中产生，发病率高达100%，通过2或3次s.c.c注射1 × 107活的同基因睾丸生殖细胞（TC），而无需借助佐剂、百日咳杆菌疫苗或其他免疫操作。第一次注射TC后第40天。病变的特点是炎症细胞间质浸润和睾丸内严重的低精子发生，导致整个器官萎缩和。相反，完全不受附睾炎的影响。免疫学研究表明，这种形式的免疫可引起对同基因TC的延迟型超敏反应和体液抗体反应。我们比较了A/J、AKR、BALB/C、C3H/He、C57BL/6和DBA/2 6种不同近交系小鼠对这种类型EAO诱导的敏感性。除DBA/2小鼠外，其余菌株均或多或少出现EAO病变，C3H/He和a /J菌株均出现严重病变的频率较高。由于该小鼠EAO模型可以在不使用弗氏完全佐剂和百日咳疫苗的情况下诱导，其本质上是单纯的“自身免疫”，可能为进一步研究调节生殖细胞抗原的有害自身免疫反应导致男性不育的免疫机制提供新的途径。
Experimental autoimmune orchitis (EAO) was produced in C3H/He mice with as high as 100% incidence by two or three s.c. injections of 1 × 107 viable syngeneic testicular germ cells (TC) without resorting to adjuvants, Bordetella pertussis vaccine, or other immunological manipulations. On day 40 after the first injection of TC. the lesions induced were characterized by interstitial infiltration of inflammatory cells and severe hypospermatogenesis in the testis with resulting whole organ atrophy and. in contrast, by a complete lack of epididymitis. Immunological studies revealed that this form of immunization caused both delayed‐type hypersensitivity and humoral antibody responses to syngeneic TC. We compared the susceptibilities to the induction of this type of EAO among six different strains of inbred mice comprising A/J, AKR, BALB/C, C3H/He, C57BL/6 and DBA/2 mice. All strains except for DBA/2 mice developed lesions of EAO to a greater or lesser extent, and severe disease was induced with high frequency in two strains, C3H/He and A/J. As this murine model of EAO can be induced without the use of Freund's complete adjuvant and B. pertussis vaccine, it is simply ‘autoimmune’ in nature and may provide new ways for further investigation into the immunological mechanisms which regulate deleterious autoimmune reactions to germ cell antigens leading to the male infertility.