Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis
Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis
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DOI：
10.1038/nplants.2015.35
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发表时间：
2015-03-30
期刊：
影响因子：
18
通讯作者：
Nishimura, Mikio
中科院分区：
文献类型：
--
作者：
Oikawa, Kazusato;Matsunaga, Shigeru;Nishimura, Mikio
地球上的生命依赖于光合作用，光合作用消耗二氧化碳并产生氧气和碳水化合物。光合作用是由植物细胞内的动态环境维持的，涉及大量具有细胞质流的细胞器。对叶绿体、线粒体和过氧化物酶体的生理研究表明，这些细胞器在光呼吸过程中积极进行通讯，光呼吸是光合作用产生的副产物被回收的过程。然而，代谢物有效交换的机制尚未明确定义。我们发现叶绿体上的过氧化物酶体的形状从球形变为椭圆形，并且它们的相互作用面积在光呼吸过程中增加。我们应用最新的飞秒激光技术来分析拟南芥叶栅栏叶肉细胞内细胞器之间的粘附，并成功地估计了它们在不同环境条件下的物理相互作用。这是这种估计方法在活细胞中的首次应用。我们的研究结果表明，光合作用依赖性相互作用在确保光呼吸过程中代谢物高效流动方面发挥着关键作用。
Life on earth relies upon photosynthesis, which consumes carbon dioxide and generates oxygen and carbohydrates. Photosynthesis is sustained by a dynamic environment within the plant cell involving numerous organelles with cytoplasmic streaming. Physiological studies of chloroplasts, mitochondria and peroxisomes show that these organelles actively communicate during photorespiration, a process by which by-products produced by photosynthesis are salvaged. Nevertheless, the mechanisms enabling efficient exchange of metabolites have not been clearly defined. We found that peroxisomes along chloroplasts changed shape from spherical to elliptical and their interaction area increased during photorespiration. We applied a recent femtosecond laser technology to analyse adhesion between the organelles inside palisade mesophyll cells of Arabidopsis leaves and succeeded in estimating their physical interactions under different environmental conditions. This is the first application of this estimation method within living cells. Our findings suggest that photosynthetic-dependent interactions play a critical role in ensuring efficient metabolite flow during photorespiration.