The Response to 2-Aminoacrylate Differs in Escherichia coli and Salmonella enterica, despite Shared Metabolic Components
The Response to 2-Aminoacrylate Differs in Escherichia coli and Salmonella enterica, despite Shared Metabolic Components
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DOI：
10.1128/jb.00140-17
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发表时间：
2017-07-01
影响因子：
3.2
通讯作者：
Downs, Diana M.
中科院分区：
文献类型：
--
作者：
Borchert, Andrew J.;Downs, Diana M.
生物体的代谢网络包括存在的生化反应的总和。在微生物中，这个网络具有无可挑剔的能力，可以感知和响应由内部或外部刺激引起的扰动。代谢潜力（即，网络结构）通常基于被认为指示特定途径的酶的存在而从基因组序列中得出。大肠杆菌和肠道沙门氏菌是革兰氏阴性菌的肠杆菌科的成员，它们共享大部分代谢组分和作为"核心基因组"的调控机制。"在S.在肠道中，已经在体内建立了烯胺中间体2-氨基丙烯酸酯（2AA）抑制许多吡哆醛5'-磷酸（PLP）依赖性酶的能力。本文研究了2AA在E.杆菌数据表明，尽管所有相关酶的保守性，S。enterica和E.大肠杆菌在2AA的产生和有害后果方面存在差异。总之，这些研究结果表明，围绕内源性2AA胁迫的产生和响应的代谢网络的结构在S. enterica和E.这项工作比较了肠杆菌科两个密切相关成员中内源性应激物2-氨基丙烯酸酯周围的代谢网络。数据显示，尽管在这个代谢节点中所有相关酶都是保守的，但这两种密切相关的生物在代谢网络结构上存在分歧。这项工作突出了一组保守的组件如何产生不同的网络架构，以及这如何影响生物体的生理。这项工作定义了一个模型，以扩大我们对2-氨基丙烯酸酯应激反应的理解，以及S. enterica和E.杆菌
The metabolic network of an organism includes the sum total of the biochemical reactions present. In microbes, this network has an impeccable ability to sense and respond to perturbations caused by internal or external stimuli. The metabolic potential (i.e., network structure) of an organism is often drawn from the genome sequence, based on the presence of enzymes deemed to indicate specific pathways. Escherichia coli and Salmonella enterica are members of the Enterobacteriaceae family of Gram-negative bacteria that share the majority of their metabolic components and regulatory machinery as the "core genome." In S. enterica, the ability of the enamine intermediate 2-aminoacrylate (2AA) to inactivate a number of pyridoxal 5'-phosphate (PLP)-dependent enzymes has been established in vivo. In this study, 2AA metabolism and the consequences of its accumulation were investigated in E. coli. The data showed that despite the conservation of all relevant enzymes, S. enterica and E. coli differed in both the generation and detrimental consequences of 2AA. In total, these findings suggest that the structure of the metabolic network surrounding the generation and response to endogenous 2AA stress differs between S. enterica and E. coli.IMPORTANCE This work compared the metabolic networks surrounding the endogenous stressor 2-aminoacrylate in two closely related members of the Enterobacteriaceae. The data showed that despite the conservation of all relevant enzymes in this metabolic node, the two closely related organisms diverged in their metabolic network structures. This work highlights how a set of conserved components can generate distinct network architectures and how this can impact the physiology of an organism. This work defines a model to expand our understanding of the 2-aminoacrylate stress response and the differences in metabolic structures and cellular milieus between S. enterica and E. coli.