IDENTIFICATION OF TUMOR STEM-LIKE CELLS IN A MOUSE MYELOMA CELL LINE
IDENTIFICATION OF TUMOR STEM-LIKE CELLS IN A MOUSE MYELOMA CELL LINE
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DOI：
10.1170/131
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发表时间：
2009-01-01
影响因子：
1.6
通讯作者：
Gu, N.
中科院分区：
文献类型：
--
作者：
Dou, J.;Li, Y.;Gu, N.
分别采用软琼脂和无血清培养基集落形成实验、BrdU标记保留细胞和SP细胞鉴定方法以及BALB/c小鼠致瘤性实验对SP2/0细胞系中的肿瘤干细胞进行分析。结果表明，少数SP2/0细胞能够在软琼脂中形成集落，并在SP2/0细胞系中含有BrdU标记的永生化链。无血清培养的SP2/0细胞在BALB/c小鼠体内的致瘤性高于完全培养的SP2/0细胞。总体而言，只有少数SP2/0细胞具有肿瘤干细胞样的特征，如高增殖能力、更多的自我更新和更强的成瘤性，或者更接近于肿瘤干细胞(TSCs)的特征。肿瘤干细胞的生物学有助于识别对未来肿瘤治疗重要的分子靶点。
We used colony formation assay in the soft agar media or the serum-free media, the methods of identifying BrdU-label-retaining cells and the SP cells as well as the tumorigenicity test in BALB/c mice, respectively, to analyze tumor stem like cells in the SP2/0 cell line. The results showed that a few SP2/0 cells were capable of forming colonies in the soft agar media, contained BrdU-label-immortal strand in the SP2/0 cell line. The SP2/0 cells in the serum-free media gained higher tumorigenicity in the BALB/c mice than the SP2/0 cells cultivated in the complete media did. Overall, only a few of the SP2/0 cells were found to possess the characteristics of tumor stem-like cells, such as high proliferative potency, more self-renewal and stronger tumorigenesis, or greater similarity to the tumor stem cells (TSCs) traits. The biology of tumor stem-like cells contributes to the identification of molecular targets important for future tumor therapy.