Concentration control for protein crystallization via a continuously-fed crystallization chamber.
Concentration control for protein crystallization via a continuously-fed crystallization chamber.
复制标题
通过连续进料结晶室控制蛋白质结晶的浓度。
DOI：
10.1039/b801686k
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发表时间：
2008
期刊：
影响因子：
6.1
通讯作者：
Barocas,VictorH
中科院分区：
文献类型：
--
作者：
Sugiyama,Masano;Sengupta,Shramik;Todd,Paul;Barocas,VictorH
一个连续进料的结晶室，允许通过结晶相图（从不稳定/成核到亚稳/生长）的动力学路径控制制造和用于结晶溶菌酶。建立了一个集总动力学模型，并确定了异相成核动力学参数。正如预期的那样，异相成核比均匀成核具有更快的成核动力学和更慢的生长动力学。新装置的主要贡献是（1）允许更好地控制晶体成核和生长研究的化学环境，以及（2）允许对这些研究进行集总模型分析以提取动力学参数。
A continuously-fed crystallization chamber that allows for kinetic path control through the crystallization phase diagram (from labile/nucleation to metastable/growth) was fabricated and used to crystallize lysozyme. A lumped kinetic model was developed, and parameters for heterogeneous nucleation kinetics were determined. Heterogeneous nucleation was found to have faster nucleation kinetics and slower growth kinetics than homogeneous nucleation, as expected. The major contributions of the new device are (1) to allow better control of the chemical environment for studies of crystal nucleation and growth, and (2) to allow lumped-model analysis of those studies to extract kinetic parameters.