Impact of Purkinje Cell Simple Spike Synchrony on Signal Transmission from Flocculus.
Impact of Purkinje Cell Simple Spike Synchrony on Signal Transmission from Flocculus.
复制标题
浦肯野细胞简单尖峰同步对小叶信号传输的影响。
DOI：
10.1007/s12311-021-01332-w
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Connor,TeneshaL
中科院分区：
文献类型：
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作者：
Stahl,JohnS;Ketting-Olivier,Aaron;Tendolkar,PrasadA;Connor,TeneshaL
小脑小叶中的浦肯野细胞（PCs）携带速率编码信息，最终驱动眼球运动。彼此相邻的絮状pc表现出部分的简单尖峰（SS）同步。在小脑的其他地方，PC - SS同步已被证明影响PC突触目标的活动，一些人认为它构成了信息传递的另一个载体。我们在小脑小叶中研究了速率码与PC同步的相互作用程度。这项研究的动机之一是解释像蹒跚这样的共济失调小鼠的小脑缺陷；我们推测PC同步对突变体中丢失的速率码传输有积极影响。在PCs表达通道视紫质的转基因小鼠中，我们利用光遗传学的特性来控制PCs同步：脉冲光刺激产生刺激锁定的峰值，而连续光刺激产生的峰值时间不受限制。我们分别用定时正弦变化的脉冲刺激和强度正弦变化的连续刺激对小叶PCs进行光激活。对PC对的记录证实，脉冲刺激产生更大的PC同步。我们从放电速率调制的幅度和眼动来量化诱发的PC放电速率调制的传输效率。在产生更大的PC同步的条件下，速率码传输稍微差一些，反驳了我们关于共济失调的起源的动机猜测。光遗传刺激显著增强了250-300 Hz的局部场电位振荡，并证明了振荡功率与诱发的PC同步之间的关系。
Purkinje cells (PCs) in the cerebellar flocculus carry rate-coded information that ultimately drives eye movement. Floccular PCs lying nearby each other exhibit partial synchrony of their simple spikes (SS). Elsewhere in the cerebellum, PC SS synchrony has been demonstrated to influence activity of the PCs’ synaptic targets, and some suggest it constitutes another vector for information transfer. We investigated in the cerebellar flocculus the extent to which the rate code and PC synchrony interact. One motivation for the study was to explain the cerebellar deficits in ataxic mice liketottering; we speculated that PC synchrony has a positive effect on rate code transmission that is lost in the mutants. Working in transgenic mice whose PCs express channelrhodopsin, we exploited a property of optogenetics to control PC synchrony: pulsed photostimulation engenders stimulus-locked spiking, whereas continuous photostimulation engenders spiking whose timing is unconstrained. We photoactivated flocculus PCs using pulsed stimuli with sinusoidally varying timing vs. continuous stimuli with sinusoidally varying intensity. Recordings of PC pairs confirmed that pulsed stimuli engendered greater PC synchrony. We quantified the efficiency of transmission of the evoked PC firing rate modulation from the amplitudes of firing rate modulation and eye movement. Rate code transmission was slightly poorer in the conditions that generated greater PC synchrony, arguing against our motivating speculation regarding the origin of ataxia intottering. Floccular optogenetic stimulation prominently augmented a 250–300 Hz local field potential oscillation, and we demonstrate relationships between the oscillation power and the evoked PC synchrony.