Dose response study of conjugated fatty acid derived from safflower oil on mammary and colon carcinogenesis pretreated with 7,12-dimethylbenz[a]anthracene (DMBA) and 1,2-dimethylhydrazine (DMH) in female Sprague-Dawley rats
Dose response study of conjugated fatty acid derived from safflower oil on mammary and colon carcinogenesis pretreated with 7,12-dimethylbenz[a]anthracene (DMBA) and 1,2-dimethylhydrazine (DMH) in female Sprague-Dawley rats
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DOI：
10.1016/s0304-3835(03)00280-5
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发表时间：
2003-07-10
期刊：
影响因子：
9.7
通讯作者：
Shirai, T
中科院分区：
文献类型：
--
作者：
Cheng, JL;Futakuchi, M;Shirai, T
为了阐明富含共轭亚油酸（CLA）的红花油共轭脂肪酸（CFA-S）对乳腺癌和结肠癌的化学预防作用，6周龄雌性Sprague-Dawley （SD）大鼠分别饲喂含有0.01、0.05的日粮。0.1、1或2% CFA-S，随后在头11天内皮下注射1,2-二甲肼(DMH) /k 5次，剂量为40mg /kg体重，单次50mg /kg体重灌胃施用7,12-二甲基苯[a]蒽（DMBA）。实验于第36周结束。分析乳腺肿瘤数量、结肠异常隐窝灶（ACF）数量、乳腺肿瘤增殖指数、结肠上皮细胞数量。1%的剂量被发现是抑制两个靶器官癌变的最佳剂量，与细胞增殖的数据之间存在良好的相关性。研究结果表明，饮食中含有适当水平的CFA-S可能有助于预防乳腺癌和结肠癌。2003爱思唯尔科学有限公司版权所有。
To clarify the chemopreventive effects of conjugated fatty acid derived from safflower oil (CFA-S), rich in conjugated linoleic acid (CLA), on mammary and colon carcinogenesis, 6 week old female Sprague-Dawley (SD) rats received diet containing 0.01, 0.05. 0.1, 1, or 2% CFA-S subsequent to five times subcutaneous injections of 1,2-dimethyl-hydrazine (DMH) /k, at a dose of 40 mg/kg b.w. and a single 50 mg/kg b.w. intragastric application of 7,12-dimethylbenz[a]anthracene (DMBA) during the first 11 days. The experiment was terminated at week 36. Numbers of mammary tumors, colon aberrant crypt foci (ACF), and proliferative indices of mammary tumors, and colon epithelium were analyzed. The 1% dose was found to be optimal for suppression of carcinogenesis in both target organs, a good correlation being noted with between data for cell proliferation. The,,e results suggest that a diet containing appropriate levels of CFA-S may be useful for prevention of mammary and colon cancer. (C) 2003 Elsevier Science Ltd. All rights reserved.