Effect of Cryopreservation on Biological and Immunological Properties of Stem Cells From Apical Papilla
Effect of Cryopreservation on Biological and Immunological Properties of Stem Cells From Apical Papilla
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DOI：
10.1002/jcp.22050
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发表时间：
2010-05-01
影响因子：
5.6
通讯作者：
Wang, Songlin
中科院分区：
文献类型：
--
作者：
Ding, Gang;Wang, Wei;Wang, Songlin
根尖乳头干细胞（Stem cells from apical papilla，SCAP）是一种新型的多能干细胞，虽然与牙髓干细胞相似，但却是牙齿干细胞的独立来源。SCAP在牙根发育、根尖发生、牙髓/牙本质再生和生物根工程中具有潜在的作用。然而，尚未探索存储和保存SCAP以供未来临床应用的程序。在这项研究中，我们比较了人类新鲜分离的SCAP（fSCAP）与冷冻保存的SCAP（cSCAP）的细胞活力，集落形成效率，细胞增殖率，多向分化潜能，间充质干细胞（MSC）标志物的配置文件，核型分析和免疫学检测。与fSCAP相比，cSCAP显示出相似的活细胞比率、集落形成效率、细胞增殖率、多向分化潜能、MSC表面标志物、凋亡率和G带核型。在免疫特性方面，fSCAP和cSCAP之间没有显著差异。此外，小型猪cSCAP具有与fSCAP相似的增殖速率、分化潜能和免疫调节功能。本研究表明，低温保存不影响SCAP的生物学和免疫学特性，支持SCAP在氮气中低温保存的可行性。J.细胞。223：415-422，2010。(C)2010 Wiley-Liss，Inc.
Stem cells from apical papilla (SCAP) are a novel population of multipotent stem cells that, although similar to dental pulp stem cells, are a discrete source of dental stem cells. SCAP have potential roles in root development, apexogenesis, pulp/dentin regeneration, and bioroot engineering. However, procedures to store and preserve SCAP for future clinical applications have not been explored. In this study, we compared human freshly isolated SCAP (fSCAP) with cryopreserved SCAP (cSCAP) in terms of cell viability, colony-forming efficiency, cell proliferation rate, multilineage differentiation potential, profiles of mesenchymal stem cell (MSC) markers, karyotype analysis, and immunological assays. cSCAP showed a similar viable cell ratio, colony-forming efficiency, cell proliferation rate, multilineage differentiation potential, MSC surface markers, apoptotic rate, and G-banded karyotype when compared to fSCAP. There was no significant difference between fSCAP and cSCAP with regard to immune properties. In addition, cSCAP of miniature pig possessed the similar proliferation rate, differentiation potential, and immunomodulatory function as seen in fSCAP. This study demonstrates that cryopreservation does not affect the biological and immunological properties of SCAP, supporting the feasibility of SCAP cryopreservation in nitrogen. J. Cell. Physiol. 223: 415-422, 2010. (C) 2010 Wiley-Liss, Inc.