The Notch ligand, Jagged-1, influences the development of primitive hematopoietic precursor cells
The Notch ligand, Jagged-1, influences the development of primitive hematopoietic precursor cells
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DOI：
10.1182/blood.v91.11.4084.411k05_4084_4091
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发表时间：
1998-06-01
期刊：
影响因子：
20.3
通讯作者：
Bernstein, ID
中科院分区：
文献类型：
--
作者：
Varnum-Finney, B;Purton, LE;Bernstein, ID
我们检测了造血细胞中Notch家族的两个成员Notch-1和Notch-2以及一种Notch配体Jagged-1的表达。在小鼠骨髓前体 (Lin(-)Sca-1(+)c-kit(+)) 中检测到了 Notch-1 和 Notch-2。 Notch 配体 Jagged-1 在全骨髓或前体细胞中均未检测到。然而，在培养的原代小鼠胎肝基质、培养的原代小鼠骨髓基质和基质细胞系中发现了 Jagged-1。这些结果表明Notch-Notch配体相互作用在造血过程中的潜在作用。为了进一步测试这种可能性，通过将分选的前体细胞 (Lin(-)Sca-1(+)c-kit(+)) 与表达人 Jagged-1 的 3T3 细胞层共培养，或将分选的前体细胞与包被有纯化的人 Jagged-1 胞外结构域 (Jagged-1(ext)) 的珠子一起孵育，评估了 Jagged-1 对小鼠骨髓前体细胞的影响。我们发现，存在于细胞表面和珠子上的 Jagged-1 促进原始前体细胞群形成的两倍至三倍。这些结果表明Notch配体在体外扩大前体细胞群中的潜在用途。 (C) 1998 年，美国血液学会。
We examined the expression of two members of the Notch family, Notch-1- and Notch-2, and one Notch ligand, Jagged-1, in hematopoietic cells. Both Notch-1 and Notch-2 were detected in murine marrow precursors (Lin(-)Sca-1(+)c-kit(+)). The Notch ligand, Jagged-1, was not detected in whole marrow or in precursors. However, Jagged-1 was seen in cultured primary murine fetal liver stroma, cultured primary murine bone marrow stroma, and in stromal cell lines. These results indicate a potential role for Notch-Notch ligand interactions in hematopoiesis. To further test this possibility, the effect of Jagged-1 on murine marrow precursor cells was assessed by coculturing sorted precursor cells (Lin(-)Sca-1(+)c-kit(+)) with a 3T3 cell layer that expressed human Jagged-1 or by incubating sorted precursors with beads coated with the purified extracellular domain of human Jagged-1 (Jagged-1(ext)). We found that Jagged-1, presented both on the cell surface and on beads, promoted a twofold to threefold increase in the formation of primitive precursor cell populations. These results suggest a potential use for Notch ligands in expanding precursor cell populations in vitro. (C) 1998 by The American Society of Hematology.