DIGITAL IMAGING OF FREE CALCIUM CHANGES AND OF SPATIAL GRADIENTS IN GROWING PROCESSES IN SINGLE, MAMMALIAN CENTRAL-NERVOUS-SYSTEM CELLS
DIGITAL IMAGING OF FREE CALCIUM CHANGES AND OF SPATIAL GRADIENTS IN GROWING PROCESSES IN SINGLE, MAMMALIAN CENTRAL-NERVOUS-SYSTEM CELLS
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DOI：
10.1073/pnas.83.16.6179
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发表时间：
1986-08-01
影响因子：
11.1
通讯作者：
CONNOR, JA
中科院分区：
文献类型：
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作者：
CONNOR, JA
用荧光指示剂Fura-2和数字成像技术测定了培养的中枢神经系统（CNS）细胞内游离钙水平。从大鼠胚胎间脑（胚胎第17或18天）中接种细胞，几乎所有存活分离的细胞在前24小时内都经历了最后的有丝分裂。最初的球形细胞在培养的前24小时内观察到，当时它们正在延伸过程，但尚未建立纤维网络，这将阻止识别给定纤维的起源。快速延伸的细胞在生长区域显示出高的Ca 2+水平。在过程刚刚出现从相同的500 nM是ca或生长是从一个以上的极进行，Ca 2+水平是均匀的，估计水平的mmonly看到。在远离索马的活性生长锥中，Ca 2+水平超过200 nM，而索马水平在60-80 nM范围内。非扩展和扩展的细胞，已停滞有统一的Ca 2+水平在30-70 nM的范围内。结果表明，高Ca 2+水平至少与CNS细胞中的延伸相关，并且在某些条件下，高钙区域可以定位于细胞的一小部分。
Intracellular free calcium levels have been measured in cultured central nervous system (CNS) cells by using the fluorescent indicator fura-2 and digital imaging techniques. Cells were plated from rat embryo diencephalon (embryonic day 17 or 18), with nearly all of the cells surviving dissocation having undergone final mitosis within the previous 24 hr. The initial spherical cells were observed within the first 24 hr of culture when they were extending processes but had not established a network of fibers that would prevent the identification of the origin of a given fiber. Cells that were rapidly extending showed high Ca2+ levels in the regions of growth. Where processes had just emerged from the same500 nM were ca or where growth was proceeding from more than one pole, Ca2+ levels were uniform and estimated levels of mmonly seen. In active growth cones distant from the soma, Ca2+ levels exceeded 200 nM, whereas the soma levels were in the 60-80 nM range. Nonextended and extended cells that had stalled had uniform Ca2+ levels in the range of 30-70 nM. The results show that high Ca2+ levels are at least a correlate of extension in CNS cells and that under some conditions the region of high calcium can be localized to a small part of the cell.