LATENT HERPES-SIMPLEX VIRUS IN HUMAN TRIGEMINAL GANGLIA - DETECTION OF AN IMMEDIATE EARLY GENE ANTISENSE TRANSCRIPT BY INSITU HYBRIDIZATION
LATENT HERPES-SIMPLEX VIRUS IN HUMAN TRIGEMINAL GANGLIA - DETECTION OF AN IMMEDIATE EARLY GENE ANTISENSE TRANSCRIPT BY INSITU HYBRIDIZATION
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DOI：
10.1056/nejm198712033172302
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发表时间：
1987-12-03
影响因子：
158.5
通讯作者：
STRAUS, SE
中科院分区：
文献类型：
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作者：
CROEN, KD;OSTROVE, JM;STRAUS, SE
我们采用原位杂交技术研究了单纯疱疹病毒1型基因在人感觉神经节潜伏感染中的表达。三叉神经节在尸检中从24名受试者中恢复，没有活动力疱疹感染的证据。这些神经节与35s标记的单链RNA探针杂交，跨越了72%的单纯疱疹基因组。在16名受试者的神经节中，0.2%至4.3%的神经细胞含有丰富的病毒RNA核信号。单纯疱疹1型抗体水平低或检测不到的3例患者的神经节缺乏病毒RNA信号，而抗体滴度升高的6例患者的神经节均显示病毒RNA信号。转录仅从病毒基因组中含有一个基因的区域检测到，该基因编码一种称为感染细胞蛋白数零（ICP0）的即时早期蛋白。然而，正常的（“感觉”）转录本，这是突出的急性感染，没有检测到。相反，一个新的转录本被发现与ICP0转录本重叠，但方向相反（因此是“反义的”）。虽然该转录本仅部分被描述，但我们相信它可能在维持单纯疱疹病毒的潜伏期中起作用。
We used in situ hybridization to study the expression of herpes simplex virus type 1 genes during latent infections of human sensory ganglia. Trigeminal ganglia were recovered at autopsy from 24 subjects with no evidence of an active herpetic infection. These ganglia were hybridized to 35S-labeled single-stranded RNA probes spanning 72 percent of the herpes simplex genome. In the ganglia of 16 subjects, 0.2 to 4.3 percent of the neuronal cells contained abundant nuclear signals for viral RNA. Ganglia from three patients with low or undetectable levels of antibodies to herpes simplex type 1 lacked viral RNA signals, whereas the ganglia from all of six patients with elevated antibody titers showed viral RNA signals. Transcription was detected only from the region of the viral genome containing a gene that encodes an immediate early protein known as the infected-cell protein number zero (ICP0). However, normal ("sense") transcripts of this gene, which are prominent in an acute infection, were not detected. In contrast, a novel transcript was found overlapping with but opposite in direction to, the ICP0 transcript (and was therefore "anti-sense"). Although this transcript has been only partially characterized, we believe that it may have a role in maintaining the latency of herpes simplex virus.