Complete Genome of the Broad-Host-Range Erwinia amylovora Phage ΦEa21-4 and Its Relationship to Salmonella Phage Felix O1
Complete Genome of the Broad-Host-Range Erwinia amylovora Phage ΦEa21-4 and Its Relationship to Salmonella Phage Felix O1
复制标题
DOI：
10.1128/aem.02352-08
复制
发表时间：
2009-04-01
影响因子：
4.4
通讯作者：
Svircev, Antonet M.
中科院分区：
文献类型：
--
作者：
Lehman, Susan M.;Kropinski, Andrew M.;Svircev, Antonet M.
已经确定了感染梨火疫病菌、梨火疫病菌和成团泛菌菌株的肌病毒噬菌体Phi Ea 21 -4的第一个完整基因组序列。这个末端冗余、环状排列的基因组的独特序列是84，576 bp。Phi Ea 21 -4基因组的GC含量为43.8%，包含117个推定的蛋白质编码基因和26个tRNA基因。Phi Ea 21 -4是第一个发现在整个基因组中分散有精确保守的rho非依赖性终止子的噬菌体，总共有24个拷贝。在Phi Ea 21 -4基因组中还值得注意的是存在具有六个和九个碱基反密码子环的tRNA，不存在小的包装末端酶亚基，以及存在nadV，NAD（+）补救途径的主要组分，迄今为止仅在少数噬菌体基因组中发现。Phi Ea 21 -4是第一个报道的Felix O 1样噬菌体基因组;预测的Phi Ea 21 -4蛋白与沙门氏菌噬菌体的蛋白有56%的同源性。除了与Felix O 1的这种相似性之外，Phi Ea 21 -4基因组似乎与先前报道的序列在全球和局部都有很大不同。117个基因中有43个是Phi Ea 21 -4所特有的，32个Felix O 1样基因不出现在除Phi Ea 21 -4和Felix O 1以外的任何噬菌体基因组序列中。N-末端测序和基质辅助激光解吸电离-飞行时间分析鉴定了5个Phi Ea 21 -4编码病毒体结构蛋白的基因，包括主要衣壳蛋白。
The first complete genome sequence for a myoviridal bacteriophage, Phi Ea21-4, infecting Erwinia amylovora, Erwinia pyrifoliae, and Pantoea agglomerans strains has been determined. The unique sequence of this terminally redundant, circularly permuted genome is 84,576 bp. The Phi Ea21-4 genome has a GC content of 43.8% and contains 117 putative protein-coding genes and 26 tRNA genes. Phi Ea21-4 is the first phage in which a precisely conserved rho-independent terminator has been found dispersed throughout the genome, with 24 copies in all. Also notable in the Phi Ea21-4 genome are the presence of tRNAs with six- and nine-base anticodon loops, the absence of a small packaging terminase subunit, and the presence of nadV, a principle component of the NAD(+) salvage pathway, which has been found in only a few phage genomes to date. Phi Ea21-4 is the first reported Felix O1-like phage genome; 56% of the predicted Phi Ea21-4 proteins share homology with those of the Salmonella phage. Apart from this similarity to Felix O1, the Phi Ea21-4 genome appears to be substantially different, both globally and locally, from previously reported sequences. A total of 43 of the 117 genes are unique to Phi Ea21-4, and 32 of the Felix O1-like genes do not appear in any phage genome sequences other than Phi Ea21-4 and Felix O1. N-terminal sequencing and matrix-assisted laser desorption ionization-time of flight analysis resulted in the identification of five Phi Ea21-4 genes coding for virion structural proteins, including the major capsid protein.