Fine mapping and identification of the leaf shape gene BoFL in ornamental kale
Fine mapping and identification of the leaf shape gene BoFL in ornamental kale
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观赏羽衣甘蓝叶形基因BoFL的精细定位与鉴定
DOI：
10.1007/s00122-020-03551-x
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发表时间：
2020-01-30
影响因子：
5.4
通讯作者：
Zhu, Pengfang
中科院分区：
文献类型：
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作者：
Feng, Xin;Li, Xin;Zhu, Pengfang
BoFL是羽衣甘蓝羽叶性状的候选基因，位于C9染色体374.532 kb区域。叶形是观赏甘蓝的一个重要经济性状。确定影响叶片形状的基因将有助于深入了解叶片发育的机制。本研究以羽衣甘蓝羽叶自交系F0819与光滑叶自交系S0835为材料，构建了F1、F2、BC1P1、BC1P2和F2:3个群体。遗传分析表明，该羽叶性状受半显性基因BoFL控制。利用大量分离分析测序，我们将BoFL基因定位在C9染色体上的4.17 mb区间。接下来，我们通过对两个侧翼标记INDEL940和INDEL5443的精细比对，将候选区域缩小到374.532 kb。我们在候选区域鉴定了38个基因，其中只有Bo9g184610在两亲本系之间表达水平存在显著差异。该基因在亲本系中测序发现3个单核苷酸多态性（SNP）差异，包含2个非同义和1个同义SNP，这导致F0819的天冬氨酸和苯丙氨酸残基编码差异，而S0835的丙氨酸和亮氨酸残基编码差异。与叶形性状共分离的第一个非同义SNP对应的cleaved扩增多态性序列（CAPS）标记CAPS4610。因此，我们推测赋予羽叶性状的基因是BoALG10，它是ALG10的同源基因，在拟南芥中编码α -1,2-葡萄糖基转移酶。该研究将有助于了解叶片发育机制，并为羽衣甘蓝新品种的选育提供重要信息。
BoFL, a candidate gene conferring the feathered-leaved trait in ornamental kale, is located in a 374.532-kb region on chromosome C9. Leaf shape is an important economic trait in ornamental kale (Brassica oleracea var. acephala). Identifying the genes that influence leaf shape would provide insight into the mechanism underlying leaf development. In this study, we constructed F1, F2, BC1P1, BC1P2, and F2:3populations from a cross between a feathered-leaved inbred line, F0819, and a smooth-leaved inbred line, S0835, of ornamental kale. Genetic analysis showed that the feathered-leaved trait was controlled by a semi-dominant gene, BoFL. Using bulked segregant analysis sequencing, we mapped the BoFL gene to a 4.17-Mb interval on chromosome C9. Next, we narrowed down the candidate region to 374.532-kb by fine-scale mapping between the two flanking markers INDEL940 and INDEL5443. We identified 38 genes in the candidate region, among which only Bo9g184610 showed significant variation in expression level between the two parental lines. Sequencing of the gene in the parental lines identified three single-nucleotide polymorphism (SNP) differences, containing two nonsynonymous and one synonymous SNPs, which resulted in coding variations of an aspartate and a phenylalanine residue in F0819, but an alanine and a leucine residue in S0835. A cleaved amplified polymorphic sequence (CAPS) marker, CAPS4610, corresponding to the first nonsynonymous SNP co-segregated with the leaf shape trait. We thus speculated that the gene conferring the feathered-leaved trait is BoALG10, a homolog of ALG10, which encodes an alpha-1,2-glucosyltransferase in Arabidopsis thaliana. This work will be useful for understanding the mechanism of leaf development and provides important information for the breeding of kale with novel leaf shapes.