Arabidopsis homologues of the autophagy protein Atg8 are a novel family of microtubule binding proteins
Arabidopsis homologues of the autophagy protein Atg8 are a novel family of microtubule binding proteins
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DOI：
10.1016/j.febslet.2004.04.088
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发表时间：
2004-06-04
期刊：
影响因子：
3.5
通讯作者：
Hussey, PJ
中科院分区：
文献类型：
--
作者：
Ketelaar, T;Voss, C;Hussey, PJ
自噬是蛋白质和多余细胞器的非选择性运输，目的是降解到真菌的液泡或动物细胞的溶酶体中。一些基因编码的自噬途径的组成部分是保守的植物，在这里，我们表明，拟南芥同源酵母Atg 8（Apg 8/Aut 7）和Atg 4（Apg 4/Aut 2）部分互补的酵母缺失菌株。酵母双突变体，Atg 8和Atg 4的缺失菌株，不能被拟南芥Atg 8互补，表明拟南芥Atg 8需要Atg 4为其功能。此外，拟南芥Atg 8和拟南芥Atg 4直接相互作用的双杂交测定。有趣的是，Atg 8与MAP 1A和B的微管结合轻链3显示出显著的同源性，并且在这里我们表明拟南芥Atg 8隐藏微管。我们的研究结果表明，在植物中的自噬途径的一个主要组成部分是类似的，在酵母中，我们认为，微管结合在这一过程中发挥了作用。(C)2004年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
Autophagy is the non-selective transport of proteins and superfluous organelles destined for degradation to the vacuole in fungae, or the lysosome in animal cells. Some of the genes encoding components of the autophagy pathway are conserved in plants, and here we show that Arabidopsis homologues of yeast Atg8 (Apg8/Aut7) and Atg4 (Apg4/Aut2) partially complement the yeast deletion strains. The yeast double mutant, a deletion strain with respect to both Atg8 and Atg4, could not be complemented by Arabidopsis Atg8, indicating that Arabidopsis Atg8 requires Atg4 for its function. Moreover, Arabidopsis Atg8 and Arabidopsis Atg4 interact directly in a two-hybrid assay. Interestingly, Atg8 shows significant homolgy with the microtubule binding light chain 3 of MAP1A and B, and here we show that Arabidopsis Atg8 hinds microtubuies. Our results demonstrate that a principle component of the autophagic pathway in plants is similar to that in yeast and we suggest that microtubule binding plays a role in this process. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.