Pyruvate oxidation in vivo and in vitro in Dictyostelium discoideum.
Pyruvate oxidation in vivo and in vitro in Dictyostelium discoideum.
复制标题
盘基网柄菌体内和体外丙酮酸氧化。
DOI：
10.1016/0304-4165(89)90125-6
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Wright,BE
中科院分区：
文献类型：
--
作者：
Butler,MH;Wright,BE
The in vivo rate of the reaction catalyzed by the pyruvate dehydrogenase complex inDictyostelium discoideumwas measured by exposing cells to [1-14C]alanine and relating the rate of14CO2evolution to the specific radioactivity of the isolated [1-14C]pyruvate. The rate obtained, 0.33 μmol/min per ml packed cell volume, was very similar to that determined by independent calculations based upon the rate of utilization of endogenous protein as an energy source in this system.
