Hendra Virus Infection Dynamics in the Grey-Headed Flying Fox (Pteropus poliocephalus) at the Southern-Most Extent of Its Range: Further Evidence This Species Does Not Readily Transmit the Virus to Horses.
Hendra Virus Infection Dynamics in the Grey-Headed Flying Fox (Pteropus poliocephalus) at the Southern-Most Extent of Its Range: Further Evidence This Species Does Not Readily Transmit the Virus to Horses.
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DOI：
10.1371/journal.pone.0155252
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wang LF
中科院分区：
文献类型：
--
作者：
Burroughs AL;Durr PA;Boyd V;Graham K;White JR;Todd S;Barr J;Smith I;Baverstock G;Meers J;Crameri G;Wang LF
亨德拉病毒（HeV）是澳大利亚的一种重要的新出现的病毒，已知在东海岸的某些地区感染马和人类。虽然狐蝠被认为是HeV的天然宿主，但四种大陆物种中哪一种是主要宿主一直是持续争论的来源，特别是因为共享栖息地是常见的。为了帮助解决这个问题，我们在灰头狐蝠（Pteropus poliocephalus）分布范围的最南端，对其中一种物种进行了采样。在两年的时间里，我们使用大约每周一次的合并尿液采样技术，使用新型多重（Luminex）平台确定了HeV和相关副粘病毒的患病率。虽然所有合并的尿液样本均为HeV核酸阴性，但我们成功鉴定了其他四种副粘病毒，包括雪松病毒;一种与HeV密切相关的亨尼帕病毒。从殖民地单独捕获的蝙蝠收集的血清显示，如通过血清学Luminex测定所估计的，HeV抗体存在于14.6%至44.5%的动物中。估计的范围很广，反映了在解释中期结果时的不确定性。解释的背景下，从国家到北方的HeV研究的研究，我们增加了支持一个正在形成的共识，这是黑狐蝠，而不是灰头狐蝠，是HeV的主要来源，在溢出事件的马。
Hendra virus (HeV) is an important emergent virus in Australia known to infect horses and humans in certain regions of the east coast. Whilst pteropid bats (“flying foxes”) are considered the natural reservoir of HeV, which of the four mainland species is the principal reservoir has been a source of ongoing debate, particularly as shared roosting is common. To help resolve this, we sampled a colony consisting of just one of these species, the grey-headed flying fox, (Pteropus poliocephalus), at the southernmost extent of its range. Using the pooled urine sampling technique at approximately weekly intervals over a two year period, we determined the prevalence of HeV and related paramyxoviruses using a novel multiplex (Luminex) platform. Whilst all the pooled urine samples were negative for HeV nucleic acid, we successfully identified four other paramyxoviruses, including Cedar virus; a henipavirus closely related to HeV. Collection of serum from individually caught bats from the colony showed that antibodies to HeV, as estimated by a serological Luminex assay, were present in between 14.6% and 44.5% of animals. The wide range of the estimate reflects uncertainties in interpreting intermediate results. Interpreting the study in the context of HeV studies from states to the north, we add support for an arising consensus that it is the black flying fox and not the grey-headed flying fox that is the principal source of HeV in spillover events to horses.