Identification of the Centrifuged Lipoaspirate Fractions Suitable for Postgrafting Survival.
Identification of the Centrifuged Lipoaspirate Fractions Suitable for Postgrafting Survival.
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适合移植后存活的离心脂肪抽吸组分的鉴定。
DOI：
10.1097/prs.0000000000001883
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发表时间：
2016
影响因子：
3.6
通讯作者：
Chenggang Yi
中科院分区：
文献类型：
--
作者：
Lihong Qiu;Yingjun Su;Dongliang Zhang;Yajuan Song;Bei Liu;Zhou Yu;Shuzhong Guo;Chenggang Yi
背景：科尔曼离心程序产生具有不同脂肪细胞和祖细胞密度的组分。本研究的目的是确定所有的馏分是可行的implantation.Methods：人脂肪抽吸物进行处理，通过科尔曼离心。将离心机任意分为上层、中层和下层。测定脂肪细胞活力、形态学、基质血管成分细胞和脂肪间充质干细胞的数量。结果：损伤的脂肪细胞主要分布在上层，而完整的脂肪细胞分布在中层和下层。在离心液中形成基质血管组分细胞密度梯度。上层、中层和下层样本的植入物体积保留率分别为33.44± 5.9%、55.11± 4.4%和71.2± 5.8%。此外，中间和下层含有更多的脂肪来源的干细胞比上层。结论：下层含有更多的活脂肪细胞和基质血管成分细胞导致最高的植入体体积保留，而最受损的细胞分布在上层，导致最少的体积保留。尽管基质血管组分含量较低，但中间层具有大量完整的脂肪细胞，这些脂肪细胞能够在植入后保留部分脂肪组织体积，这表明当需要大量脂肪移植物进行移植时，中间层可能是替代脂肪来源。
Background:The Coleman centrifugation procedure generates fractions with different adipocyte and progenitor cell densities. This study aimed to identify all fractions that are feasible for implantation.Methods:Human lipoaspirates were processed by Coleman centrifugation. The centrifugates were divided arbitrarily into upper, middle, and lower layers. Adipocyte viability, morphology, numbers of stromal vascular fraction cells, and adipose-derived mesenchymal stem cells of each layer were determined. The 12-week volume retention of subcutaneously implanted 0.3-ml lipoasperate of each layer was investigated in an athymic mice model.Results:Most damaged adipocytes were located in the upper layers, whereas the intact adipocytes were distributed in the middle and lower layers. A gradient of stromal vascular fraction cell density was formed in the centrifugates. The implant volume retentions of samples from the upper, middle, and lower layers were 33.44±5.9, 55.11±4.4, and 71.2±5.8 percent, respectively. Furthermore, the middle and lower layers contained significantly more adipose-derived stem cells than did the upper layer.Conclusions:The lower layer contains more viable adipocytes and stromal vascular fraction cells leading to the highest implant volume retention, whereas the most impaired cells are distributed in the upper layer, leading to the least volume retention. Although with a lower stromal vascular fraction content, the middle layer has a substantial number of intact adipocytes that are capable of retaining partial adipose tissue volume after implantation, suggesting that the middle layer may be an alternative fat source when large volumes of fat grafts are needed for transplantation.