Effect of traditional Chinese medicine berberine on type 2 diabetes based on comprehensive metabonomics
Effect of traditional Chinese medicine berberine on type 2 diabetes based on comprehensive metabonomics
复制标题
基于综合代谢组学的中药小檗碱对2型糖尿病的影响
DOI：
10.1016/j.talanta.2010.01.015
复制
发表时间：
2010-05-15
期刊：
影响因子：
6.1
通讯作者：
Ning, Guang
中科院分区：
文献类型：
--
作者：
Gu, Yan;Zhang, Yifei;Ning, Guang
采用代谢组学方法，结合指纹图谱分析和靶点分析，研究小檗碱治疗2型糖尿病合并血脂异常的作用机制。收集了60例患者接受黄连素或安慰剂治疗前后的血清样本。超高效液相色谱法和四极杆飞行时间串联质谱法（UPLC Q-TOF MS）结合模式识别分析用于鉴定总体血清代谢物的变化。与安慰剂相比，黄连素治疗前后的患者可以分为不同的集群所显示的正交信号校正过滤偏最小二乘判别分析（OSC-PLS-DA）评分图，这表明黄连素治疗后循环代谢物的变化。其中，游离脂肪酸变化明显。通过UPLC结合单四极杆质谱法（UPLC SQ MS）进一步定量。黄连素给药后，13种脂肪酸的浓度显著降低。10种脂肪酸也与安慰剂存在统计学差异。这些结果表明，小檗碱可能发挥关键作用，在治疗2型糖尿病，通过下调高水平的游离脂肪酸和全面的代谢组学测量是潜在的非常有用的研究中药的作用机制。(C)2010 Elsevier B. V.保留所有权利。
A comprehensive metabonomic method, in combination with fingerprint analysis and target analysis, was performed to reveal potential mechanisms of berberine action in the treatment of patients with type 2 diabetes and dyslipidemia. Serum samples of 60 patients before and after treatment with either berberine or placebo were collected. Ultra-performance liquid chromatography and quadrupole time-of-flight tandem mass spectrometry (UPLC Q-TOF MS) coupled with pattern recognition analysis were used to identify changes in global serum metabolites. Compared with placebo, patients before and after berberine treatment could be separated into distinct clusters as displayed by the orthogonal signal correction filtered partial least-squares discriminant analysis (OSC-PLS-DA) score plot, which indicated changes in circulating metabolites after berberine treatment. Among them, free fatty acids changed markedly. These were further quantified by UPLC combined with single quadrupole mass spectrometry (UPLC SQ MS). There was a highly significant decrease in the concentrations of 13 fatty acids following berberine administration. 10 fatty acids also differed statistically from placebo. These results suggest that berberine might play a pivotal role in the treatment of type 2 diabetes through down-regulating the high level of free fatty acids and that comprehensive metabonomic measurements are potentially very useful for studying the mechanisms of action of traditional Chinese medicines. (C) 2010 Elsevier B.V. All rights reserved.