Biofilm formation, epiphytic fitness, and canker development in Xanthomonas axonopodis pv citri
Biofilm formation, epiphytic fitness, and canker development in Xanthomonas axonopodis pv citri
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DOI：
10.1094/mpmi-20-10-1222
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发表时间：
2007-10-01
影响因子：
3.5
通讯作者：
Marano, Maria Rosa
中科院分区：
文献类型：
--
作者：
Rigano, Luciano A.;Siciliano, Florencia;Marano, Maria Rosa
植物病原细菌Xanthomonaxonopodispv.柑橘是影响全世界柑橘植物的溃疡病的罪魁祸首。在这里，我们已经评估了细菌附着和生物膜形成的作用，在柠檬叶片溃疡病的发展过程中，在叶片定殖。结晶紫染色和共聚焦激光扫描显微镜分析。轴足虫变种使用表达绿色荧光蛋白的柑橘属菌株来评价在非生物和生物（叶）表面上的附着和生物膜形成。野生型X.轴足虫变种柑橘在含有葡萄糖的基本培养基中附着于玻璃上并在玻璃上形成复杂的结构化生物膜。在柠檬叶上也观察到类似的附着和结构化生物膜形成。X轴足虫变种在胞外多糖黄原胶的生产中有缺陷的citri gumB突变菌株在非生物或生物表面上都不形成结构化的生物膜。此外，X.轴足虫变种柑桔胶B在叶表面上表现出降低的生长和存活以及减轻的疾病症状。这些结果表明，生物膜的形成在X的附生生存的重要作用。轴足虫变种柑橘溃疡病的发展之前。
The phytopathogenic bacterium Xanthomonas axonopodis pv. citri is responsible for the canker disease affecting citrus plants throughout the world. Here, we have evaluated the role of bacterial attachment and biofilm formation in leaf colonization during canker development on lemon leaves. Crystal violet staining and confocal laser scanning microscopy analysis of X. axonopodis pv. citri strains expressing the green fluorescent protein were used to evaluate attachment and biofilm formation on abiotic and biotic (leaf) surfaces. Wild-type X. axonopodis pv. citri attached to and formed a complex, structured biofilm on glass in minimal medium containing glucose. Similar attachment and structured biofilm formation also were seen on lemon leaves. An X. axonopodis pv. citri gumB mutant strain, defective in production of the extracellular polysaccharide xanthan, did not form a structured biofilm on either abiotic or biotic surfaces. In addition, the X. axonopodis pv. citri gumB showed reduced growth and survival on leaf surfaces and reduced disease symptoms. These findings suggest an important role for formation of biofilms in the epiphytic survival of X. axonopodis pv. citri prior to development of canker disease.