The TBP-like factor CeTLF is required to activate RNA polymerase II transcription during C-elegans embryogenesis
The TBP-like factor CeTLF is required to activate RNA polymerase II transcription during C-elegans embryogenesis
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DOI：
10.1016/s1097-2765(00)00068-x
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发表时间：
2000-09-01
期刊：
影响因子：
16
通讯作者：
Mango, SE
中科院分区：
文献类型：
--
作者：
Kaltenbach, L;Horner, MA;Mango, SE
后生动物具有两种TATA结合蛋白同源物，一般转录因子TBP和称为TLF的相关因子。TLF在RNA聚合酶II（Pol II）转录中的作用有四种模型：（1）TLF和TBP功能冗余，（2）TLF拮抗TBP，（3）TLF是组织特异性TBP，（4）TLF和TBP具有不同的活性。在这里，我们报告说，CeTLF需要表达Pol II基因的一个子集，并与至少一个这些基因在体内。CeTLF也是在早期胚胎发生过程中建立批量转录所必需的。由于CeTLF和CeTBP在相同的细胞中以相当的水平表达，这些发现表明CeTLF在激活Pol II转录中执行不同于CeTBP的独特功能。
Metazoans possess two TATA-binding protein homologs, the general transcription factor TBP and a related factor called TLF. Four models have been proposed for the role of TLF in RNA polymerase II (Pol Il) transcription: (1) TLF and TBP function redundantly, (2) TLF antagonizes TBP, (3) TLF is a tissue-specific TBP, or (4) TLF and TBP have distinct activities. Here we report that CeTLF is required to express a subset of Pol II genes and associates with at least one of these genes in vivo. CeTLF is also necessary to establish bulk transcription during early embryogenesis. Since CeTLF and CeTBP are expressed at comparable levels in the same cells, these findings suggest CeTLF performs a unique function in activating Pol II transcription distinct from that of CeTBP.