Brain cytokine and chemokine mRNA expression in mice induced by intranasal instillation with ultrafine carbon black
Brain cytokine and chemokine mRNA expression in mice induced by intranasal instillation with ultrafine carbon black
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DOI：
10.1016/j.toxlet.2005.10.006
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发表时间：
2006-05-25
影响因子：
3.5
通讯作者：
Fujimaki, H
中科院分区：
文献类型：
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作者：
Tin-Tin-Win-Shwe;Yamamoto, S;Fujimaki, H
环境空气超细颗粒（ufp）引起了许多研究人员的极大关注，最近有证据表明，它们对人体健康的危害比较大的环境颗粒更大。关注对大脑影响可能性的研究相当有限。为了研究超细炭黑（ufCB）对小鼠脑的影响，我们将14nm或95nm的超细炭黑125 μ g滴入8周龄雄性BALB/c小鼠鼻孔，每周1次，连续4周。末次灌胃4 h后，采集大鼠嗅球和海马，采用实时荧光定量PCR法检测细胞因子和趋化因子mRNA的表达。在本研究中，我们发现经鼻灌胃14 nm ufCB小鼠的脑嗅球中诱导了促炎细胞因子（白细胞介素-1 β和肿瘤坏死因子- α）和趋化因子（单核细胞趋化蛋白-1/CCL2，巨噬细胞炎性蛋白-1 α /CCL3），以及单因子诱导的干扰素- γ /CXC趋化因子配体（CXCL9） mRNA，而不是海马。我们认为鼻内注射ufCB可能会影响脑免疫功能，这取决于它们的大小。据我们所知，这是第一个在生理相关条件下，通过鼻内注射特定大小的ufCB触发小鼠区域特异性脑细胞因子和趋化因子mrna诱导的研究。2005爱思唯尔爱尔兰有限公司版权所有。
Ambient air ultrafine particles (UFPs) have gained enormous attention to many researchers with recent evidence showing them to have more hazardous effects on human health than larger ambient particles. Studies focusing the possibility of effects on brain are quite limited. To examine the effect of ultrafine carbon black (ufCB) on mice brain, we instilled 125 mu g of 14 nm or 95 nm CB into the nostrils of 8-week-old male BALB/c mice, once a week for 4 weeks. Four hours after the last instillation, we collected olfactory bulb and hippocampus and detected the expression of cytokine and chemokine mRNA by quantitative real-time PCR method. In this study, we found the induction of proinflammatory cytokines (interleukin-1 beta and tumor necrosis factor-alpha and chemokines (monocyte chemoattractant protein-1/CCL2, macrophage inflammatory protein-1 alpha/CCL3), and monokine induced interferon-gamma/CXC chemokine ligand (CXCL9) mRNA in brain olfactory bulb, not in the hippocampus of mice instilled with 14 nm ufCB intranasally. We suggest that the intranasal instillation of ufCB may influence the brain immune function depending on their size. To our knowledge, this is the first study to demonstrate region-specific brain cytokine and chemokine mRNA-induction in mice triggered by intranasal instillation of specific-sized ufCB, in a physiologically relevant condition. (c) 2005 Elsevier Ireland Ltd. All rights reserved.