The foveal avascular region of developing human retina
The foveal avascular region of developing human retina
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DOI：
10.1001/archopht.126.4.507
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发表时间：
2008-04-01
影响因子：
--
通讯作者：
Hendrickson, A. E.
中科院分区：
文献类型：
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作者：
Provis, J. M.;Hendrickson, A. E.
目的：研究视网膜中央凹周围血管的发育。方法：对7只孕26周至41周的视网膜进行研究。切片使用高分辨率数字摄影和以200%至300%的放大倍数识别的血管轮廓进行成像。用CD31和第VIII因子抗体免疫标记血管和视紫红质抗体以识别无视杆细胞区。结果：中心凹区域无视杆感光细胞，位于视网膜外部和/或视网膜内有浅凹陷。26周时，整个坐骨台呈无血管区，以无杆状区为中心，沿颞侧水平经线开放。妊娠37周时，黄斑中心凹无血管区形成一个完整的环。在切片上，胎龄35周的黄斑缺血区直径约为500 pm，孕40周的黄斑缺血区直径约为300~350 pm，在整个胎座上，37周和41周的黄斑缺血区直径约为150~170 pm。妊娠数周。结论：黄斑中心凹区域在发育过程中通常是无血管的，就像成年人一样。我们在人类视网膜发育过程中没有发现黄斑中心凹血管形成的证据。临床相关性：黄斑中心凹区域血管形成的例子并不是由于一过性血管系统的失败退化所致。
Objective: To study the development of the perifoveal retinal vasculature.Methods: We studied 7 retinas aged between 26 weeks' gestation and I week postnatal (41 weeks' gestation). Sections were imaged using high-resolution digital photography and blood vessel profiles identified at 200% to 300% magnification. Flat mounts were immunolabeled using antibodies to CD31 and factor VIII to identify blood vessels and antibodies to rhodopsin to identify the rod-free zone.Results: The foveal region was identified by the absence of rod photoreceptors; in the outer retina and/or presence of a shallow depression in the inner retina. The whole mount at 26 weeks' gestation showed a blood vessel-free region centered on the rod-free zone that was open along the horizontal meridian on the temporal side. At 37 weeks' gestation, the foveal avascular zone formed a complete circle. In sections, the foveal avascular zone was approximately 500 pm in diameter at 35 weeks' gestation and 300 to 350 pm at 40 weeks' gestation; in whole mounts, it was 150 to 170 pm in diameter at 37 and 41. weeks' gestation.Conclusions: The foveal region is normally avascular during development, as in adult life. We found no evidence of foveal vascularization during development of the human retina.Clinical Relevance: Instances of vascularization of the foveal region are not due to failed regression of a transient vasculature.