A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants
A Resource for Inactivation of MicroRNAs Using Short Tandem Target Mimic Technology in Model and Crop Plants
复制标题
在模型植物和作物植物中使用短串联靶模拟技术灭活 MicroRNA 的资源
DOI：
10.1016/j.molp.2018.09.003
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发表时间：
2018-11-05
期刊：
影响因子：
27.5
通讯作者：
Tang, Guiliang
中科院分区：
文献类型：
--
作者：
Peng, Ting;Qiao, Mengmeng;Tang, Guiliang
microRNA (miRNA) 是内源性小非编码 RNA，可与 mRNA 结合并靶向它们进行切割和/或翻译抑制，从而导致基因沉默。我们之前开发了短串联靶模拟 (STTM) 技术来灭活拟南芥中的内源 miRNA。在这里，我们创建了数百个 STTM，它们针对拟南芥、番茄、水稻和玉米中的保守和物种特异性 miRNA，为 miRNA 的功能询问提供了资源。我们不仅揭示了几种 miRNA 在植物发育中的功能，而且还证明了水稻中一些 miRNA 的组织特异性失活会导致籽粒尺寸增加，而不会对整个植物的生长和发育产生不利影响。 STTM156/157和STTM165/166转基因植物的RNA-seq和小RNA-seq分析揭示了这些miRNA在植物激素生物合成和激活、次生代谢和离子通道活性相关电生理学中的作用，表明STTM技术是研究miRNA功能的有效方法。为了促进STTM转基因植物的研究和应用，并为存储和共享miRNA调控基因网络的信息提供有用的平台，我们建立了在线基因组浏览器（https://blossom.ffr.mtu.edu/designindex2.php）来显示STTM诱导的miRNA敲低植物中的转录组和miRNAomic变化。
microRNAs (miRNAs) are endogenous small non-coding RNAs that bind to mRNAs and target them for cleavage and/or translational repression, leading to gene silencing. We previously developed short tandem target mimic (STTM) technology to deactivate endogenous miRNAs in Arabidopsis. Here, we created hundreds of STTMs that target both conserved and species-specific miRNAs in Arabidopsis, tomato, rice, and maize, providing a resource for the functional interrogation of miRNAs. We not only revealed the functions of several miRNAs in plant development, but also demonstrated that tissue-specific inactivation of a few miRNAs in rice leads to an increase in grain size without adversely affecting overall plant growth and development. RNA-seq and small RNA-seq analyses of STTM156/157 and STTM165/166 transgenic plants revealed the roles of these miRNAs in plant hormone biosynthesis and activation, secondary metabolism, and ion-channel activity-associated electrophysiology, demonstrating that STTM technology is an effective approach for studying miRNA functions. To facilitate the study and application of STTM transgenic plants and to provide a useful platform for storing and sharing of information about miRNA-regulated gene networks, we have established an online Genome Browser (https://blossom.ffr.mtu.edu/designindex2.php) to display the transcriptomic and miRNAomic changes in STTM-induced miRNA knockdown plants.