Generation of Expressed Sequence Tags of Random Root cDNA Clones of Brassica napus by Single-Run Partial Sequencing
Generation of Expressed Sequence Tags of Random Root cDNA Clones of Brassica napus by Single-Run Partial Sequencing
复制标题
通过单次部分测序生成甘蓝型油菜随机根 cDNA 克隆的表达序列标签
DOI：
10.1104/pp.103.2.359
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发表时间：
1993
影响因子：
6
通讯作者：
Hong Gil Nam
中科院分区：
文献类型：
--
作者：
Yu Shin Park;June M. Kwak;O;Yong Sung Kim;Dae Sil Lee;Moo Je Cho;Hyung Hoan Lee;Hong Gil Nam
通过对197个随机根cDNA克隆的单次部分测序，获得了237个表达序列标签（est）。计算机检索结果显示，21个ESTs与现有数据库中的蛋白质编码序列具有显著的相似性，其中包括5个与胁迫或防御相关的基因和4个与其他基因相关的克隆。对10个数据库匹配的cDNA克隆进行Northern blot分析，发现许多克隆在根中表达最丰富，而在其他器官中表达较少。然而，两个无性系具有高度的根特异性。结果表明，通过随机cDNA克隆的部分测序和表达分析生成根系ESTs是一种大规模分离植物根系功能基因的有效方法。我们还讨论了cDNA文库的检测结果和适合这种方法的测序方法。
Two hundred thirty-seven expressed sequence tags (ESTs) of Brassica napus were generated by single-run partial sequencing of 197 random root cDNA clones. A computer search of these root ESTs revealed that 21 ESTs show significant similarity to the protein-coding sequences in the existing data bases, including five stress- or defense-related genes and four clones related to the genes from other kingdoms. Northern blot analysis of the 10 data base-matched cDNA clones revealed that many of the clones are expressed most abundantly in root but less abundantly in other organs. However, two clones were highly root specific. The results show that generation of the root ESTs by partial sequencing of random cDNA clones along with the expression analysis is an efficient approach to isolate genes that are functional in plant root in a large scale. We also discuss the results of the examination of cDNA libraries and sequencing methods suitable for this approach.
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs
DOI：
10.1073/pnas.84.13.4355
发表时间：
1987-07-01
影响因子：
11.1
作者：
GRIBSKOV, M;MCLACHLAN, AD;EISENBERG, D
通讯作者：
EISENBERG, D